199664

Sudan Black B

certified by the Biological Stain Commission

• Sinonimo/i: Ceres black BN, Fat Black HB, Solvent Black 3
• Formula empirica (notazione di Hill): C₂₉H₂₄N₆
• Numero CAS: 4197-25-5
• Peso molecolare: 456.54
• Colour Index: 26150
• Beilstein: 723248
• Numero CE: 224-087-1
• Numero MDL: MFCD00006919
• Codice UNSPSC: 12171500
• ID PubChem: 24851932
• NACRES: NA.47

Proprietà

• Grado: certified by the Biological Stain Commission
• Livello qualitativo: 200
• Forma fisica: powder
• Colore: dark brown to very dark brown
• Punto di fusione: 120-124 °C (lit.)
• λmax: 415 nm (2nd); 598 nm
• ε (coefficiente d’estinzione): ≥20000 at 596-603 nm in ethanol
• applicazioni: diagnostic assay manufacturing; hematology; histology
• Temperatura di conservazione: room temp
• Stringa SMILE: CC1(C)Nc2cccc3c(ccc(N1)c23)\N=N\c4ccc(\N=N\c5ccccc5)c6ccccc46
• InChI: 1S/C29H24N6/c1-29(2)30-26-14-8-13-22-25(17-18-27(31-29)28(22)26)35-34-24-16-15-23(20-11-6-7-12-21(20)24)33-32-19-9-4-3-5-10-19/h3-18,30-31H,1-2H3/b33-32+,35-34+; YCUVUDODLRLVIC-VPHDGDOJSA-N

Descrizione

Applicazioni

Sudan Black B has been used in brain BLAQ (Block Lipids and Aldehyde Quench) technique and is used in lipid detection.

Azioni biochim/fisiol

Sudan Black B has been used to stain phospholipids and intracellular lipids. Staining pattern is almost similar to myeloperoxidase reaction, with positive staining of granulocytic cells, eosinophils and weak monocytic staining.It doesn′t stain lymphocytes. Sudan Black B can be used to stain older blood or bone marrow smears, but the stain slightly fades over time. It is considered to be a most sensitive and specific reagent for lipid staining. Sudan Black B stains phospholipids gray and neutral fats blue-black.

Compatibilità

Certified as a fat staining using the Churukian variant of the Lillie-Ashburn supersaturated isopropanol method. Sudan III is substituted for the oil red O in the procedure. Burdon′s stain for bacterial lipid.

Informazioni sulla sicurezza

• Pittogrammi: GHS08
• Avvertenze: Warning
• Indicazioni di pericolo: H341
• Consigli di prudenza: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Classi di pericolo: Muta. 2
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)