11585029001

Blu nitrotetrazolo cloruro

crystals, pkg of 5 g

• Sinonimo/i: 2,2′-bis(4-nitrofenil)-5,5′-difenil-3,3′-(3,3′-dimetossi-4,4′-difenilene)ditetrazolo cloruro, 3,3′-(3,3′-dimetossi-4,4′-bifenilene)bis[2-(4-nitrofenil)-5-fenil-2H-tetrazolo cloruro], p-blu nitrotetrazolo, p-blu nitrotetrazolo cloruro, NBT, Nitro BT
• Formula condensata: C₄₀H₃₀N₁₀O₆ · 2Cl
• Numero CAS: 298-83-9
• Peso molecolare: 817.64
• Beilstein: 4115923
• Numero MDL: MFCD00012159
• Codice UNSPSC: 41116100
• ID PubChem: 329749491

Proprietà

• Descrizione: 4-Nitro blue tetrazolium chloride, crystals
• Livello qualitativo: 100
• Forma fisica: crystals
• Confezionamento: pkg of 5 g
• Produttore/marchio commerciale: Roche
• Temperatura di conservazione: 2-8°C
• Stringa SMILE: [Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8
• InChI: 1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2; FSVCQIDHPKZJSO-UHFFFAOYSA-L

Descrizione

Descrizione generale

NBT is soluble in water, aqueous buffers, and dimethylformamide.

Tetrazolium salts are useful in determining the metabolic activity of cells from many origins. The quaternary tetrazole ring core containing four nitrogen atoms, with a net positive charge induces cellular uptake through plasma membrane potential. This drives its use in cell biology. In immunolabeling technique, 4-Nitro blue tetrazolium (NBT) serves as an oxidant. The hydrolysis of 5-bromo-4-chloro-3-indolyl phosphate (BCIP) by alkaline phosphatase gives rise to leucoindigo, which is then being reduced by NBT to form an insoluble blue BCI. Certain cells reduce NBT, which is a colorless/yellow dye to blue or black formazan crystals. Due to this property, NBT is prefered in redox histochemistry and in biochemical applications. It is formerly used to distinguish between nonbacterial and bacterial diseases, the latter causing neutrophils to reduce the dye; used to confirm diagnosis of chronic granulomatous disease.

Applicazioni

• Colony and plaque hybridization
• Immunohistocytochemistry
• In situ hybridization
• Northern blot
• Southern blot
• Western blot

NBT has been used in in situ hybridization.

Qualità

Performance-controlled in the LDH assay.

Nota sulla preparazione

Working solution: Solubility: soluble in water, aqueous buffers, and dimethylformamide. Can be dissolved in methanol. A clear solution is achieved at 20 mg/ml.

Risultati analitici

Absorption: NBT has its maximum absorption at 260 nm [in water],
and the final product formazan has its maximum absorption at 605 nm [in nitrobenzole].

Altre note

For life science research only. Not for use in diagnostic procedures.

Informazioni sulla sicurezza

• Pittogrammi: GHS07
• Avvertenze: Warning
• Indicazioni di pericolo: H302,H315,H319,H335
• Consigli di prudenza: P261 - P264 - P280 - P304 + P340 + P312 - P337 + P313 - P403 + P233
• Classi di pericolo: Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Organi bersaglio: Respiratory system
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): does not flash
• Punto d’infiammabilità (°C): does not flash