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Key Documents

11051482001

Roche

dTTP

Lithium salt

Sinonimo/i:

Thymidine 5′-triphosphate sodium salt, 2′-deoxy-thymidine-5′-triphosphate, deoxy-thymidine-5′-triphosphate, 2′-, TTP, dTTP

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About This Item

Formula empirica (notazione di Hill):
C10H16N2O14P3Na
Numero CAS:
Peso molecolare:
504.15
Numero MDL:
Codice UNSPSC:
41116100
ID PubChem:

Livello qualitativo

Saggio

≥98%

Forma fisica

solution

impiego

sufficient for 6,250 standard reactions (PCR)

Confezionamento

pkg of 250 μL (25 μmol)

Produttore/marchio commerciale

Roche

Concentrazione

100 mM

tecniche

DNA amplification: suitable
DNA sequencing: suitable

Temperatura di conservazione

−20°C

InChI

1S/C10H17N2O14P3.Na.H/c1-5-3-12(10(15)11-9(5)14)8-2-6(13)7(24-8)4-23-28(19,20)26-29(21,22)25-27(16,17)18;;/h3,6-8,13H,2,4H2,1H3,(H,19,20)(H,21,22)(H,11,14,15)(H2,16,17,18);;
KAELIXTVSANDSE-UHFFFAOYSA-N

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Descrizione generale

dTTP is a 100 mM clear, colorless solution of lithium salt (pH 7).
dTTP solution in water (pH 7), 100 mM, lithium salt.

Applicazioni

Use dTTP, lithium salt, for DNA sequencing, labeling, and all types of amplification reactions or primer-extension reactions.
It has been used in PCR fingrprinting and PCR techniques.

Qualità

Typical analysis:
  • ≥98% dTTP (HPLC), ≤1.5% dTDP (HPLC), ≤0.5% dTMP (HPLC)
  • Absorbance determination A250/A260, A280/A260, A290/A260
  • DNase activity negative, according to the current Quality Control procedures
  • Suitability for PCR

Definizione di unità

1 μmol dTTP = 9.5 A260units

Altre note

For life science research only. Not for use in diagnostic procedures.

Our PCR Grade deoxynucleotides are even purer than the lithium salts, to ensure excellent performance in your PCRs. PCR Grade deoxynucleotides contain the sodium salt of the nucleotide in PCR Grade Water.
For your convenience, some of our deoxynucleotides are also available in:
  • nucleotide sets (all 4 nucleotides in separate vials)
  • ready-to-use nucleotide mixes (premixed solutions of all 4 nucleotides)

They are also vital components of:
  • kits (dNTPs, buffer, and polymerase in separate vials)
  • ready-to-use mixes [polymerase, nucleotides, and buffer (premixed 2x solutions)]

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

No data available

Punto d’infiammabilità (°C)

No data available


Certificati d'analisi (COA)

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Journal of clinical microbiology, 42(8), 3853-3856 (2004-08-07)
An immunochromatographic strip test, Xenostrip-Tv, was compared to wet mount and PCR for the diagnosis of Trichomonas vaginalis infection in women. Of 428 specimens tested, 54 (12.6%) were positive by an "expanded gold standard," defined as either a positive wet
Wieland Meyer et al.
Emerging infectious diseases, 9(2), 189-195 (2003-02-27)
A network was established to acquire basic knowledge of Cryptococcus neoformans in IberoAmerican countries. To this effect, 340 clinical, veterinary, and environmental isolates from Argentina, Brazil, Chile, Colombia, Mexico, Peru, Venezuela, Guatemala, and Spain were typed by using M13 polymerase
Luciana Trilles et al.
Memorias do Instituto Oswaldo Cruz, 103(5), 455-462 (2008-09-18)
The molecular types of 443 Brazilian isolates of Cryptococcus neoformans and Cryptococcus gattii were analyzed to determine their geographic distribution within Brazil and their underlying host conditions. The following data, imported from previous epidemiological studies as well as two culture

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