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Key Documents

11051440001

Roche

dATP

=98%, solution, 100 mM, suitable for DNA sequencing, suitable for DNA amplification

Sinonimo/i:

2′-Deoxyadenosine 5′-triphosphate sodium salt solution, 2′-deoxy-adenosine-5′-triphosphate, deoxy-adenosine-5′-triphosphate, 2′-, dATP

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About This Item

Codice UNSPSC:
41116100

Livello qualitativo

Descrizione

Lithium salt

Saggio

98%

Forma fisica

solution

impiego

sufficient for 6,250 standard reactions (PCR)

Confezionamento

pkg of 250 μL (25 μmol; 100 mM)

Produttore/marchio commerciale

Roche

Concentrazione

100 mM

tecniche

DNA amplification: suitable
DNA sequencing: suitable
DNA synthesis: suitable
PCR: suitable

Temperatura di conservazione

−20°C

Stringa SMILE

[Na+].Nc1ncnc2n(cnc12)[C@H]3C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)([O-])=O)O3

InChI

1S/C10H16N5O12P3.Na/c11-9-8-10(13-3-12-9)15(4-14-8)7-1-5(16)6(25-7)2-24-29(20,21)27-30(22,23)26-28(17,18)19;/h3-7,16H,1-2H2,(H,20,21)(H,22,23)(H2,11,12,13)(H2,17,18,19);/q;+1/p-1/t5-,6+,7+;/m0./s1
YJWCICGGRLOGEH-VWZUFWLJSA-M

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Descrizione generale

2′-Deoxyadenosine 5′-triphosphate (dATP) is made up of a nucleobase attached to deoxyribose and a 5′-hydroxyl on the sugar bound to a chain of three phosphate residues. dATP is used by cells for synthesis of DNA by DNA polymerases.
dATP is a 100 mM clear, colorless solution of the lithium salt (pH 7).Download the CofA, SDS and IFU s

Applicazioni

2′-Deoxyadenosine 5′-triphosphate sodium salt solution is used in DNA sysnthesis reactions such as PCR, DNA sequencing and molecular cloning techniques.
Use dATP, lithium salt, for DNA sequencing, labeling, and all types of amplification reactions or primer-extension reactions.
dATP has been used to resume transcription.

Azioni biochim/fisiol

Deoxyadenosine-5-triphosphate (dATP) plays a major role in the initiation of apoptosis in vitro. dATP is considered as an inhibitor of mammalian ribonucleotide reductase, thereby DNA synthesis.

Qualità

Typical analysis:
  • ≤98% dATP (HPLC), ≤1.5% dADP (HPLC), ≤0.5% dAMP (HPLC)
  • Absorbance determination A250/A260, A280/A260, A290/A260
  • DNase activity negative, according to the current Quality Control procedures
  • Suitability for PCR

Definizione di unità

1 μmol dATP = 15.0 A260 units

Altre note

For life science research only. Not for use in diagnostic procedures.
Our PCR Grade deoxynucleotides are even purer than the lithium salts, to ensure excellent performance in your PCRs. PCR Grade deoxynucleotides contain the sodium salt of the nucleotide in PCR Grade Water.
For your convenience, some of our deoxynucleotides are also available in:
  • nucleotide sets (all 4 nucleotides in separate vials)
  • ready-to-use nucleotide mixes (premixed solutions of all 4 nucleotides)
They are also vital components of:
  • kits (dNTPs, buffer, and polymerase in separate vials)
  • ready-to-use mixes [polymerase, nucleotides, and buffer (premixed 2x solutions)]

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

No data available

Punto d’infiammabilità (°C)

No data available


Certificati d'analisi (COA)

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Mfd Dynamically Regulates Transcription via a Release and Catch-Up Mechanism
Le TT, et al.
Cell (2018)
Stable suppression of the R2 subunit of ribonucleotide reductase by R2-targeted short interference RNA sensitizes p53(-/-) HCT-116 colon cancer cells to DNA-damaging agents and ribonucleotide reductase inhibitors
Lin ZP, et al.
The Journal of Biological Chemistry, 279(26), 27030-27038 (2004)
Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c
Liu X, et al.
Cell, 86(1), 147-157 (1996)

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