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10838039001

Roche

Fibronectina

from human plasma

Sinonimo/i:

Fibronectin

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
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About This Item

Numero MDL:
MFCD00131062
Codice UNSPSC:
12352207

Origine biologica

human plasma

Livello qualitativo

100

Sterilità

non-sterile; 0.2 μm filtered

Saggio

95% (SDS-PAGE)

Stato

lyophilized (clear, colorless solution after reconstitution)

PM

440 kDa

Confezionamento

pkg of 5 mg

Produttore/marchio commerciale

Roche

Condizioni di stoccaggio

avoid repeated freeze/thaw cycles

tecniche

cell culture | mammalian: suitable

N° accesso UniProt

P02751

Temperatura di conservazione

2-8°C

Informazioni sul gene

human ... FN1(2335)

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Categorie correlate

Descrizione generale

Fibronectin is responsible for the cellular interactions with the extracellular matrix. It is involved in cell adhesion, migration, growth and differentiation. It is a soluble component of plasma and other body fluids. In addition, it is also part of the insoluble extracellular matrix.

Specificità

Active on most mammalian cells

Applicazioni

Fibronectin promotes the attachment and subsequent spreading of many cells. It is used for the coating of culture dishes.

Metodo analitico

Tested for the promotion of adherence of 3T3 (NR6) cells (XTT cleavage)

Stato fisico

Lyophilizate (contains 1.126 mg glycine and 0.058 mg sodium chloride per mg fibronectin), before lyophilization filtered through a 0.2 μm pore size membrane.

Nota sulla preparazione

Working concentration: 5 μg/cm2
5 μg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.

Ricostituzione

Add 1 or 5 ml sterile water, respectively, to yield a final concentration of 1 mg/ml. Incubate for 30 to 60 minutes at 37 °C to dissolve. Do not agitate.
Upon reconstitution, the solution may contain a small amount of insoluble aggregated material. These aggregates are a phenomenon inherent to fibronectin and do not influence product performance.

Altre note

For life science research only. Not for use in diagnostic procedures.

Esclusione di responsabilità

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

does not flash

Punto d’infiammabilità (°C)

does not flash

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Certificati d'analisi (COA)

Lot/Batch Number
66091400
67434100
72438200
63148700
64326400

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Articoli

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.

3D Cell Culture Tools and Techniques

3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models

Extracellular Matrix Proteins and Tools for Cell Culture Optimization

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Protocolli

Fibronectin Coating Protocol

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.

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