10799050001
Roche
BM-Cyclin
lyophilized, active toward mycoplasma
Sinonimo/i:
antibiotic, minocycline, tiamulin
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About This Item
Codice UNSPSC:
12352207
Prodotti consigliati
Stato
lyophilized
Livello qualitativo
Confezionamento
pkg of 37.5 mg (for 2 x 2.5 l medium)
Produttore/marchio commerciale
Roche
Spettro attività antibiotica
mycoplasma
Modalità d’azione
protein synthesis | interferes
Temperatura di conservazione
2-8°C
Descrizione generale
BM-Cyclin is a combination of two antibiotics, namely tiamulin and minocycline, both of which prevent protein synthesis.
Specificità
Sensitive Organisms: Mycoplasmas and bacteria
Note: These mycoplasma strains account for more than 85% of the contaminations in animal cell cultures.
- BM Cyclin was found to effectively eliminate Acholesplasma laidlawii,
- Mycoplasma arginini,
- Mycoplasma hyorhinis, and
- Mycoplasma orale
Note: These mycoplasma strains account for more than 85% of the contaminations in animal cell cultures.
Applicazioni
BM-Cyclin is used for the elimination of mycoplasma from infected cell cultures without obvious marked cytotoxic side effects.
Confezionamento
Set of two different antibiotics
Stato fisico
Vial 1 (Tiamulin-hydrogenfumarat): clear, colorless solution after reconstitution
Vial 2 (Minocyclinhydrochloride): clear, yellow solution after reconstitution
Vial 2 (Minocyclinhydrochloride): clear, yellow solution after reconstitution
Nota sulla preparazione
Working concentration: 10μg/ml pleuromutilin derivative (BM-cyclin 1), 5μg/ml tetracycline derivative (BM-cyclin 2).
Note: These concentrations do not affect the growth of most cells. Lower concentrations may be used for sensitive cell lines.
For hybridomas decrease concentration of BM-Cyclin by 50%.
Preparation of DAPI Working Solution
Dilute the stock solution with methanol to a final concentration of 1μg/ml.
Working solution: Preparation of Stock Solution
Dissolve content of BM Cyclin 1 and BM Cyclin 2 in 10ml sterile PBS or water.
Note: These are 250x concentrated stock solutions and can be stored at -15 to -25°C for at least 6 months.Preparation of DAPI Stock Solution
Dissolve DAPI in water to a final concentration of 1 to 5mg/ml.
Note: Do not use any buffers.
This stock solution can be stored at -15 to -25°C. It is recommended to prepare appropriate aliquots.
Storage conditions (working solution): -15 to -25°C
Reconstituted stable at -15 to -25°C for at least 6 months.
Note: These concentrations do not affect the growth of most cells. Lower concentrations may be used for sensitive cell lines.
For hybridomas decrease concentration of BM-Cyclin by 50%.
Preparation of DAPI Working Solution
Dilute the stock solution with methanol to a final concentration of 1μg/ml.
Working solution: Preparation of Stock Solution
Dissolve content of BM Cyclin 1 and BM Cyclin 2 in 10ml sterile PBS or water.
Note: These are 250x concentrated stock solutions and can be stored at -15 to -25°C for at least 6 months.Preparation of DAPI Stock Solution
Dissolve DAPI in water to a final concentration of 1 to 5mg/ml.
Note: Do not use any buffers.
This stock solution can be stored at -15 to -25°C. It is recommended to prepare appropriate aliquots.
Storage conditions (working solution): -15 to -25°C
Reconstituted stable at -15 to -25°C for at least 6 months.
Altre note
For life science research only. Not for use in diagnostic procedures.
Solo come componenti del kit
N° Catalogo
Descrizione
- BM-Cyclin 1: pleuromutilin derivative
- BM-Cyclin 2: tetracycline derivative
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Organi bersaglio
Respiratory system
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
nwg
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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I clienti hanno visto anche
Hsuan Jung et al.
Chang Gung medical journal, 26(4), 250-258 (2003-07-09)
Mycoplasmas, the smallest and simplest prokaryotes that reside in endosomes of mammalian cells, are widespread contaminants found in cell cultures. About 30% of all cell cultures, varying from 15 to 80%, are reportedly contaminated with mycoplasmas. Here, we present our
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.