10102547001

AMP-PNP

Adenylyl-imidodiphosphate

• Sinonimo/i: Adenosine 5′-(β,γ-imido)triphosphate lithium salt hydrate, β,γ-Imidoadenosine 5′-triphosphate lithium salt hydrate, AMP-PNP, ATP[β,γ-NH], Adenylyl imidodiphosphate lithium salt hydrate, App(NH)p
• Formula empirica (notazione di Hill): C₁₀H₁₇N₆O₁₂P₃ · xLi⁺ · yH₂O
• Numero CAS: 25612-73-1
• Peso molecolare: 506.20 (free acid basis)
• Beilstein: 6047109
• Codice UNSPSC: 12352204

Proprietà

• Forma fisica: powder
• PM: M_r 506.2 (ANP-PNP); M_r 529.9 (AMP-PNP-Li₄)
• Confezionamento: pkg of 25 mg
• Produttore/marchio commerciale: Roche
• Stringa SMILE: [Li+].[Li+].[Li+].[Li+].[H]O[H].Nc1ncnc2n(cnc12)[C@@H]3O[C@H](COP([O-])(=O)OP([O-])(=O)NP([O-])([O-])=O)[C@@H](O)[C@H]3O
• InChI: 1S/C10H17N6O12P3/c11-8-5-9(13-2-12-8)16(3-14-5)10-7(18)6(17)4(27-10)1-26-31(24,25)28-30(22,23)15-29(19,20)21/h2-4,6-7,10,17-18H,1H2,(H,24,25)(H2,11,12,13)(H4,15,19,20,21,22,23)/t4-,6-,7-,10-/m1/s1; PVKSNHVPLWYQGJ-KQYNXXCUSA-N

Descrizione

Descrizione generale

AMP-PNP (adenylyl-imidodiphosphate) is a non-hydrolysable analogue of ATP. It inhibits fast axonal transport and facilitates the interaction between membranous organelles and microtubules.

AMP-PNP(adenylyl-imidodiphosphate) is an artificial substrate of enzyme, adenylate cyclase. Adenylate cyclase helps to hydrolyze adenylyl-imidodiphosphate slowly than ATP.

Applicazioni

AMP-PNP has been used in the preparation of nucleotide solutions, two-motor fluorescence assay, preparation of flow-cells with kinesin and cascade binding assays.

AMP-PNP is a competitive inhibitor of most ATP-dependent systems. A good substrate for enzymes hydrolyzing between the α- and β-phosphorus atom, yet is resistant to enzymes cleaving between the β- and γ-phosphorus atom. Substrate of snake venom phosphodiesterase and adenylate cyclase.
AMP-PNP has been used for two-motor fluorescence assay.

Caratteristiche e vantaggi

Contents
88% AMP-PNP (from N), 4.5% lithium, 6% water

Qualità

Contaminants: ≤1.5% Pi , <0.1% ATP (enzymatically)

Stoccaggio e stabilità

Store at -15–-25 °C. (Store dry!)

Altre note

For life science research only. Not for use in diagnostic procedures.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable