NHPMHMAG-45K
MILLIPLEX® Non-Human Primate Metabolic Magnetic Bead Panel - Metabolism Multiplex Assay
The analytes available for this multiplex kit are: Amylin (Active), C-Peptide, Ghrelin (Active), GIP, Glucagon Like Peptide-1, Glucagon, IL-6, Insulin, Leptin, MCP-1, PP, PYY.
About This Item
Prodotti consigliati
Livello qualitativo
Reattività contro le specie
nonhuman primates
Produttore/marchio commerciale
Milliplex®
assay range
accuracy: 102%
(Ghrelin (Active))
accuracy: 91%
(Insulin)
accuracy: 93%
(GLP-1)
accuracy: 94%
(PP)
accuracy: 98%
(MCP-1)
accuracy: 99%
(Leptin)
sensitivity: 0.6-65.1 pg/mL
standard curve range: 13.7-10,000 pg/mL
(Ghrelin (Active), GLP-1, Glucagon, IL-6, MCP-1, PP, PYY)
standard curve range: 137.2-100,000 pg/mL
(Insulin, Leptin)
standard curve range: 2.7-2,000 pg/mL
(GIP)
standard curve range: 27.4-20,000 pg/mL
(Amylin)
standard curve range: 68.6-50,000 pg/mL
(C-Peptide)
inter-assay cv: <15%
intra-assay cv: <10%
tecniche
multiplexing: suitable
Metodo di rivelazione
fluorometric (Luminex xMAP)
Condizioni di spedizione
wet ice
Descrizione generale
MILLIPLEX® Non-Human Primate Metabolic Panel is a 12-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, tissue/cell lysate and culture supernatant samples: Amylin (active), C-Peptide, Ghrelin (active), GIP (total), GLP-1 (active), Glucagon, IL-6, Insulin, Leptin, MCP-1/CCL2, Pancreatic Polypeptide (PP), and PYY (total). This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Metabolism
Specificità
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Applicazioni
- Analytes: Amylin (Active), C-Peptide, Ghrelin (Active), GIP (Total), Glucagon Like Peptide-1 (GLP-1) (Active), Glucagon, IL-6, Insulin, Leptin, MCP-1 (CCL2), Pancreatic Polypeptide (PP), PYY (Total)
- NOTE: When measuring specific analytes, protease inhibitors should be added to blood samples upon collection: DPPIV inhibitor for GLP-1; Protease Inhibitor Cocktail for Amylin; Aprotinin for Glucagon; Serine Protease Inhibitor for Ghrelin (Active)
- Recommended Sample Type: Non-human primate serum, plasma, cell/tissue culture supernatants and lysates.
- Recommended Sample Dilution: 25 μL per well of undiluted serum or plasma; cell/tissue culture samples may require dilution with an appropriate control medium.
- Assay Run Time: Overnight (18-20 hours) at 2-8°C.
- Research Category: Metabolism
- Research Category: Metabolic Diseases, Obesity
Caratteristiche e vantaggi
Confezionamento
Stoccaggio e stabilità
Note legali
Esclusione di responsabilità
Certificati d'analisi (COA)
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Multiplex immunoassays, such as MILLIPLEX® multiplex metabolic assays, are critical in metabolic syndrome research because they provide a full picture of the different conditions related to it, like diabetes and obesity, as well as save time and sample volume.
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