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Key Documents

MABT1512

Sigma-Aldrich

Anti-Podoplanin Antibody, clone 8.1.1

clone 8.1.1, from hamster(Syrian)

Sinonimo/i:

Glycoprotein 38, Gp38, OTS-8, PA2.26 antigen, Transmembrane glycoprotein E11, E11

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

hamster (Syrian)

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

8.1.1, monoclonal

Reattività contro le specie

mouse

Confezionamento

antibody small pack of 25 μg

tecniche

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

N° accesso NCBI

N° accesso UniProt

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

mouse ... Pdpn(14726)

Descrizione generale

Podoplanin (UniProt: Q62011; also known as Glycoprotein 38, Gp38, OTS-8, PA2.26 antigen, T1-alpha, T1A, Transmembrane glycoprotein E11) I encoded by the Pdpn (also known as Gp38) gene (Gene ID: 14726) in murine species. Podoplanin serves as the endogenous ligand of C-type lectin-like receptor-2 (CLEC-2) and is highly expressed in various tumors and in some normal cells, such as lymphatic endothelial cells and podocytes. Podoplanin is detected at high levels in lung and brain, at lower levels in kidney, stomach, liver, spleen and esophagus, and not detected in skin and small intestine. It is expressed in epithelial cells of choroid plexus, ependyma, glomerulus and alveolus, in mesothelial cells and in endothelia of lymphatic vessels. It is also expressed in stromal cells of peripheral lymphoid tissue and thymic epithelial cells and is detected in carcinoma cell lines and cultured fibroblasts. High expression of Podoplanin is observed in colon carcinomas. It may be involved in cell migration and/or actin cytoskeleton organization. Podoplanin is localized to actin-rich microvilli and plasma membrane projections, such as filopodia, lamellipodia, and ruffles. Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity. Podoplanin levels are down-regulated by treatment with puromycin aminonucleoside. Mice lacking or with defective podoplanin die at birth of respiratory failure due to a low number of attenuated type I cells, narrow and irregular air spaces, and defective formation of alveolar saccules. Administration of PMab-1 antibody is shown to reduce lymphangiogenesis in the corneal suture and ear wound healing murine models. It suppresses thioglycollate-induced macrophages infiltration at the site of wound healing. (Ref.: Maruyama, Y et al (2014). Invest.Ophthal. Visual Sci. 55(8); 4813-4822).

Specificità

Clone 8.1.1

Immunogeno

Epitope: extracellular domain
Murine thymic stromal cell line TE-7.

Applicazioni

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Podoplanin in mouse lung tissue sections.

Flow Cytometry Analysis: A representative lot detected Podoplanin in Flow Cytometry applications (Farr, A., et. al. (1992). J Histochem Cytochem. 40(5):651-64).

Immunohistochemistry Analysis: A representative lot detected Podoplanin in Immunohistochemistry applications (Farr, A., et. al. (1992). J Histochem Cytochem. 40(5):651-64).

Immunocytochemistry Analysis: A representative lot detected Podoplanin in Immunocytochemstry applications (Kamo, N., et. al. (2006). Am J Physiol Gastrointest Liver Physiol. 292(2):G526-34; Cohen, J.N., et. al. (2010). J Exp Med. 207(4):681-8; Ishii, T., et. al. (2010). Cell Tissue Res. 339(3):505-12).

Western Blotting Analysis: A representative lot detected Podoplanin in Western Blotting applications (Farr, A., et. al. (1992). J Histochem Cytochem. 40(5):651-64).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Podoplanin in Immunoprecipitation applications (Farr, A., et. al. (1992). J Histochem Cytochem. 40(5):651-64).
Research Category
Cell Structure
This syrian hamster monoclonal Anti-Podoplanin, clone 8.1.1, Cat. No. MABT1512, is a syrian hamster monoclonal antibody that detects murine Podoplanin and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Qualità

Evaluated by Immunohistochemistry (Paraffin) in mouse lung tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution of this antibody detected Podoplanin in mouse lung tissue sections.

Descrizione del bersaglio

18.23 kDa calculated.

Stato fisico

Format: Purified
Protein G purified
Purified syrian hamster monoclonal antibody IgG2-IgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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