MABS2066

Anti-LLGL1 Antibody, clone mAb17-35

clone mAb17-35, from mouse

• Sinonimo/i: Lethal(2) giant larvae protein homolog 1, Mgl-1, Mlgl
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: mAb17-35, monoclonal
• Reattività contro le specie: mouse, human
• Confezionamento: antibody small pack of 25 μg
• tecniche: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotipo: IgG2bκ
• N° accesso NCBI: NP_001152877.1
• N° accesso UniProt: Q80Y17
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: mouse ... Llgl1(16897)

Descrizione

Descrizione generale

Lethal(2) giant larvae protein homolog 1 (UniProt: Q80Y17; also known as LLGL, Mgl-1, Mlgl) is encoded by the Llgl1 (also known as Llglh) gene (Gene ID: 16897) in murine species. LLGL belongs to the WD repeat L(2)GL family. LLGL is a cortical cytoskeleton protein that is found in a complex involved in maintenance of cell polarity and epithelial integrity. It is involved in the regulation of mitotic spindle orientation, proliferation, differentiation, and tissue organization of neuroepithelial cells. It is generally enriched in developing axons. LLGL stability and function is achieve by its interaction with RanBPM protein, which primarily acts as a scaffolding protein. Its N-terminal region containing SPRY domain is shown to be responsible for binding to RanBPM and this binding functionally extends its half-life by preventing its turnover through ubiquitin-proteasomal pathway. RanBPM is shown enhance tumor suppressor activity of LLGL by inhibiting LLGL-mediated cell proliferation and cell migration. (Ref.: Suresh, B., et al. (2010). J. Biol. Chem. 285(46); 35340-35349).

Specificità

Clone mAb17-35 specifically detects LLGL1 in mouse. It targets an epitope with in 153 amino acids from the C-terminal region.

Immunogeno

Epitope: C-terminus

GST-tagged recombinant fragment corresponding to 153 amino acids from the C-terminal region of murine Lethal(2) giant larvae protein homolog 1 (LLGL).

Applicazioni

Anti-LLGL1, clone mAb17-35, Cat. No. MABS2066, is a mouse monoclonal antibody that detects Lethal(2) giant larvae protein homolog 1 and has been tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.

Research Category
Cell Structure

Western Blotting Analysis: A representative lot detected LLGL1 in Western Blotting applications (Ivanov, A.I., et. al. (2010). Am J Pathol. 176(1):134-45).

Immunocytochemistry Analysis: A representative lot detected LLGL1 in Immunocytochemistry applications (Ivanov, A.I., et. al. (2010). Am J Pathol. 176(1):134-45).

Immunohistochemistry Analysis: A representative lot detected LLGL1 in Immunohistochemistry applications (Weimer, J.M., et. al. (2009). Development. 136(17):2965-75).

Qualità

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 4 µg/mL of this antibody detected LLGL1 in mouse brain tissue lysate.

Descrizione del bersaglio

~120 kDa observed; 112.62 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Stato fisico

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.