MABN832
Anti-pan-AMPA receptor (GluR1-4), clone 2D8 Antibody
clone 2D8, from mouse
Sinonimo/i:
Glutamate receptor 1, GluR-1, AMPA-selective glutamate receptor 1, GluR-A, GluR-K1, Glutamate receptor ionotropic, AMPA 1, GluA1
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Forma dell’anticorpo
purified antibody
Tipo di anticorpo
primary antibodies
Clone
2D8, monoclonal
Reattività contro le specie
human, rat
tecniche
ELISA: suitable
electron microscopy: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
Isotipo
IgG2bκ
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... GRIA1(2890)
Descrizione generale
Glutamate receptors are synaptic receptors located primarily on the membranes of neuronal cells. Upon ligand stimulation, ionotropic glutamate receptors (iGluRs) form non-selective cation channels, while metabotropic glutamate receptors (mGluRs) indirectly activate ion channels via G protein-mediated signaling. AMPA receptors are iGluRs and are also known as alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors, AMPARs, or quisqualate receptors. AMPARs are composed of four types of subunits, GluR1/GluA1 (UniProt P42261) is encoded by GRIA1, GluR2/GluA2 (UniProt P42262) by GRIA2, GluR3/GluA3 (UniProt P42263) by GRIA3, and GluR4/GluA4 (UniProt P48058) by GRIA4 gene in human. AMPARs are tetrameric in nature, consisting of two sets of homodimers, most often a set of GluR2 dimer is coupled with a set of GluR1, GluR3 or GluR4 homodimer. Cat. No. Anti-pan-AMPA receptor (GluR1-4), clone 2D8, reacts with all four AMPA receptor subunits.
Immunogeno
Fusion protein coding for the extracellular domain of AMPA Glutamate receptor 1-4 corresponding to human pan-AMPA receptor (GluR1-4).
Applicazioni
Anti-pan-AMPA receptor (GluR1-4), clone 2D8 Antibody is an antibody against AMPA receptor for use in Western Blotting, Immunohistochemistry and Immunocytochemistry, Enzyme Immunoassay (ELISA), Electron Microscopy and Immunofluorescence.
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected AMPA receptor (GluR1-4) in 10 µg of human brain tissue lysate.
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected AMPA receptor (GluR1-4) in 10 µg of transfected human GluA1 HEK293, transfected rat GluA1 HEK293, transfected rat GluA2 HEK293, transfected rat GluA3 HEK293, and Mock transfected cell lysate.
Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected AMPA receptor (GluR1-4) in human cerebral cortex, human thalamus, rat cerebellum, and rat cerebral cortex tissue.
Immunocytochemistry Analysis: A representative lot detected AMPA receptor (GluR1-4) in Hela cells transfected with GluA1, GluA2, GluA3 and GluA4 (Courtesy of Dr John Morrison, Mount Sinai School of Medicine).
Immunofluorescence Analysis: A representative lot detected AMPA receptor (GluR1-4) in rodent, primate and human brain sections (Courtesy of Dr John Morrison, Mount Sinai School of Medicine).
Electron Microscopy Analysis: A representative lot detected AMPA receptor (GluR1-4) in rat hippocampus (Courtesy of Dr John Morrison, Mount Sinai School of Medicine).
ELISA Analysis: A representative lot detected AMPA receptor (GluR1-4) in TrpE fusion proteins (Courtesy of Dr John Morrison, Mount Sinai School of Medicine).
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected AMPA receptor (GluR1-4) in 10 µg of transfected human GluA1 HEK293, transfected rat GluA1 HEK293, transfected rat GluA2 HEK293, transfected rat GluA3 HEK293, and Mock transfected cell lysate.
Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected AMPA receptor (GluR1-4) in human cerebral cortex, human thalamus, rat cerebellum, and rat cerebral cortex tissue.
Immunocytochemistry Analysis: A representative lot detected AMPA receptor (GluR1-4) in Hela cells transfected with GluA1, GluA2, GluA3 and GluA4 (Courtesy of Dr John Morrison, Mount Sinai School of Medicine).
Immunofluorescence Analysis: A representative lot detected AMPA receptor (GluR1-4) in rodent, primate and human brain sections (Courtesy of Dr John Morrison, Mount Sinai School of Medicine).
Electron Microscopy Analysis: A representative lot detected AMPA receptor (GluR1-4) in rat hippocampus (Courtesy of Dr John Morrison, Mount Sinai School of Medicine).
ELISA Analysis: A representative lot detected AMPA receptor (GluR1-4) in TrpE fusion proteins (Courtesy of Dr John Morrison, Mount Sinai School of Medicine).
Qualità
Evaluated by Western Blotting in rat brain tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected AMPA receptor (GluR1-4) in 10 µg of rat brain tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected AMPA receptor (GluR1-4) in 10 µg of rat brain tissue lysate.
Descrizione del bersaglio
~85 kDa observed. Uncharacterized band(s) may appear in some lysates.
Stato fisico
Format: Purified
Altre note
Concentration: Please refer to lot specific datasheet.
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Raccomandato
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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