MABF3178

Anti-IL-22 Antibody, clone 9F2.2.4

• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 200
• Coniugato: unconjugated
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies, primary antibodies
• Clone: 9F2.2.4, monoclonal
• PM: calculated mol wt 20.01 kDa; observed mol wt ~17 kDa
• Purificato mediante: using protein G
• Reattività contro le specie: human
• Confezionamento: antibody small pack of 100 μg
• tecniche: immunofluorescence: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• Isotipo: IgG1κ
• Sequenza dell’epitopo: Unknown
• N° accesso ID proteina: NP_065386.1?
• N° accesso UniProt: Q9GZX6
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... IL22(50616)

Descrizione

Descrizione generale

Interleukin-22 (UniProt: Q9GZX6; also known as IL-22, Cytokine Zcyto18, IL-10-related T-cell-derived-inducible factor, IL-TIF) is encoded by the IL22 (also known as ILTIF, ZCYTO18, UNQ3099/PRO10096) gene (Gene ID: 50616) in human. IL-22 is an -helical cytokine that contributes to the inflammatory response in vivo. It is a secretory protein that is synthesized with a signal peptide (aa 1-33), which is subsequently cleaved off to produce the mature protein. It is immediately produced to initiate immune response against several tissue impairments. It is considered as a fundamental mediator in inflammation, mucous production, protective role against pathogens, wound healing, and tissue regeneration. It is produced by several populations of immune cells, including macrophages, β T cells classes Th1, Th22, and Th17 cells at the site of inflammation. It is shown to up-regulate a number of mucus associated genes, such as MUC1, MUC3, MUC10, and MUC13 in colonic and tracheal epithelial cells. Along with IL-17A, it also produces pulmonary mucus as a control against Gram negative pathogens. Its biological activity is initiated by binding to a cell-surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein (IL-22BP). IL-22 is shown to have a very high affinity for binding to IL-22BP, which acts as a natural antagonist of IL-22. IL-22BP binding occurs at specific sites on IL-22 that are also utilized by IL-22R1. (Ref.: Arshad, T., et al. (2020). Front. Immunol. 11; 2148).

Specificità

Clone 9F2.2.4 is a mouse monoclonal antibody that detects human IL-22.

Immunogeno

A full-length mature human IL-22 (without the signal peptide).

Applicazioni

Quality Control Testing

Evaluated by Western Blotting with recombinant human IL-22.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant human IL-22.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected IL-22 in human tonsil tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-IL-22, clone 9F2.2.4, Cat. No. MABF3178, is a mouse monoclonal antibody that detects human IL-22 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Stato fisico

Purified mouse monoclonal antibody IgG1 in PBS with 0.05% sodium azide.

Stoccaggio e stabilità

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable