MABF294

Anti-LAG3 Antibody, clone 8C7.1

culture supernatant, clone 8C7.1, from mouse

• Sinonimo/i: Lymphocyte activation gene 3 protein, LAG-3, Protein FDC, CD223, LAG3
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: culture supernatant
• Tipo di anticorpo: primary antibodies
• Clone: 8C7.1, monoclonal
• Reattività contro le specie: human
• tecniche: flow cytometry: suitable; western blot: suitable
• Isotipo: IgG2aκ
• N° accesso NCBI: NP_002277
• N° accesso UniProt: P18627
• Condizioni di spedizione: dry ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... LAG3(3902)

Descrizione

Descrizione generale

Lymphocyte activation gene 3 protein (UniProt P18627; also known as CD223, LAG-3, Protein FDC) is encoded by the LAG3 (also known as FDC) gene (Gene ID 3902) in human. LAG3 is a single-pass type I membrane protein that binds MHC class II molecules on antigen presenting cells with high affinity. LAG3 expression is induced after T cell activation and functions as a negative regulator of T cell function. LAG3 blockage is shown to augment T cell proliferation and cytokine production, and LAG3-deficient mice have a defect in the downregulation of T cell responses induced by staphylococcal enterotoxin B or by virus infection. In addition, LAG3 is expressed on activated regulatory T (Treg) cells at higher levels than on activated effector T cells. LAG3 blockage inhibits the suppressive activity of Treg cells. LAG3 is also upregulated on tumour-infiltrating lymphocytes (TILs), and LAG3 blockage can enhance anti-tumour T cell responses.

Immunogeno

Mouse Fc-tagged recombinant human LAG3 extracellular fragment.

Applicazioni

Flow Cytometry Analysis: A 1:100 dilution of this antibody from a representative lot detected LAG3 in 1X10E6 Jurkat cells subjected to CD3/CD28 costimulation.

This Anti-LAG3 Antibody, clone 8C7.1 is validated for use in Western Blotting, Flow Cytometry for the detection of LAG3.

Qualità

Evaluated by Western Blotting in NK-92c ell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected LAG3 in 10 µg of NK-92 cell lysate.

Descrizione del bersaglio

~60 kDa observed

Stato fisico

Mouse monoclonal IgG2aκ antibody tissue culture supernatant with 0.05% sodium azide.

Altre note

Concentration: Please refer to lot specific datasheet.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2