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Key Documents

MABF146C

Sigma-Aldrich

Anti-CD45 (Mouse), PE-Cy7, clone 30-F11 Antibody

clone 30-F11, from rat, PE-Cy7

Sinonimo/i:

Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen (L-CA), Lymphocyte antigen 5 (Ly-5), T200, CD45, CD45

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.44

Origine biologica

rat

Livello qualitativo

Coniugato

PE-Cy7

Forma dell’anticorpo

purified antibody

Tipo di anticorpo

primary antibodies

Clone

30-F11, monoclonal

Reattività contro le specie

mouse

Confezionamento

antibody small pack of 25 μg

tecniche

flow cytometry: suitable

Isotipo

IgG2bκ

N° accesso UniProt

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

mouse ... Ptprc(19264)

Descrizione generale

The 30-F11 antibody reacts with mouse CD45, which is one of the most abundant hematopoietic markers and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). CD45 is a protein tyrosine phosphatase existing in several isoforms, each being generated and expressed in cell-specific patterns. With its broad cell distribution, CD45 is critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA, CD45RB, CD45RO and others. The 30-F11 antibody is widely used as a leukocyte marker for B cells, T cell subsets and NK cell subsets.

Immunogeno

Immunogen information for this antibody is proprietary information.

Applicazioni

Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
This rat monoclonal Anti-CD45 Antibody (Mouse), PE-Cy7, clone 30-F11, Cat. No. MABF1465 is validated for use in Flow Cytometry, for the detection of CD45.

Qualità

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.125 μg from a representative lot detected CD45 in one million C57Bl/6 splenocytes.

Stato fisico

Purified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
Purified by size-exclusion chromatography

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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