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Key Documents

MABC576

Sigma-Aldrich

Anti-HOIL-1 Antibody, clone 2E2

clone 2E2, from mouse

Sinonimo/i:

RanBP-type and C3HC4-type zinc finger-containing protein 1, HBV-associated factor 4, Heme-oxidized IRP2 ubiquitin ligase 1, HOIL-1, Hepatitis B virus X-associated protein 4, RING finger protein 54, Ubiquitin-conjugating enzyme 7-interacting protein 3, HO

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.43

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

2E2, monoclonal

Reattività contro le specie

mouse, human

tecniche

immunocytochemistry: suitable
western blot: suitable

Isotipo

IgG1κ

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... RBCK1(10616)

Descrizione generale

RanBP-type and C3HC4-type zinc finger-containing protein 1 (UniProt Q9BYM8; also known as HOIL-1, HBV-associated factor 4, Heme-oxidized IRP2 ubiquitin ligase 1, Hepatitis B virus X-associated protein 4, RBCC protein interacting with PKC1, RING finger protein 54, Ubiquitin-conjugating enzyme 7-interacting protein 3) is encoded by the RBCK1 gene (also known as C20orf18, HOIL1, PBMEI, RBCK2, RNF54, UBCE7IP3, XAP3, XAP4, ZRANB4) (Gene ID 10616) in human. The linear ubiquitin assembly complex (LUBAC) is comprised of two RING-IBR-RING (RBR)-containing E3 ligases, HOIL-1L and HOIP. LUBAC conjugates a head-to-tail-linked linear polyubiquitin chain onto lysine residues on substrate proteins. Ovarian tumor deubiquitinating enzyme (DUB) with linear linkage specificity (OTULIN/Gumby/FAM105B) and cylindromatosis (CYLD) are two DUBs that catalyze the cleavage linear polyubiquitin chains. OTULIN and CYLD interact with the N-terminal PNGase/UBA or UBX (PUB) domain of HOIP and negatively regulate LUBAC-mediated cellular processes. LUBAC is reported to suppress IFN-dependent antiviral activity by mediating TRIM25 degradation and competing against TRIM25 for RIG-I interaction.

Specificità

Clone 2E2 reacts with isoform 1 (HOIL-1L) and isoform 2, but not isoform 3.

Immunogeno

Epitope: Near C-terminus
GST-tagged recombinant protein corresponding to human HOIL-1 near the C-terminus.

Applicazioni

Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
This Anti-HOIL-1 Antibody, clone 2E2 is validated for use in Western Blotting, Immunocytochemistry for the detection of HOIL-1 .
Western Blotting Analysis: A representative lot detected HOIL-1 in HeLa, SH-SY5Y, and U20S cells (Kirisako, T., et al (2006). EMBO J. 25(20):48877-4887).
Western Blotting Analysis: A representative lot detected HOIL-1 in mouse liver extracts (Tokunaga, F., et al (2009). Nat. Cell Biol. 11(2):123-132).
Western Blotting Analysis: A representative lot detected HOIL-1 in MEFs (Takiuchi, T., et al (2014). Genes Cells. 19(3):254-272).
Immunocytochemistry Analysis: A representative lot detected HOIL-1 in HeLa cells with or without Sendai virus (SeV) infection (Inn, K.S., et al. (2011). Mol Cell. 41(3):354-365).

Qualità

Evaluated by Western Blotting in THP-1 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected HOIL-1 in 10 µg of THP-1 cell lysate.

Descrizione del bersaglio

~57 kDa observed

Stato fisico

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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A ubiquitin ligase complex assembles linear polyubiquitin chains.
Kirisako, T; Kamei, K; Murata, S; Kato, M; Fukumoto, H; Kanie, M; Sano, S; Tokunaga et al.
The Embo Journal null
Involvement of linear polyubiquitylation of NEMO in NF-kappaB activation.
Tokunaga, F; Sakata, S; Saeki, Y; Satomi, Y; Kirisako, T; Kamei, K; Nakagawa, T; Kato et al.
Nature Cell Biology null
Pathogen blocks host death receptor signalling by arginine GlcNAcylation of death domains.
Li, S; Zhang, L; Yao, Q; Li, L; Dong, N; Rong, J; Gao, W; Ding, X; Sun, L; Chen, X; Chen, S; Shao, F
Nature null
Hirotsugu Oda et al.
Frontiers in immunology, 10, 479-479 (2019-04-03)
Background: HOIP is the catalytic subunit of the linear ubiquitination chain assembly complex (LUBAC) that is essential for NF-κB signaling and thus proper innate and adaptive immunity. To date only one patient with HOIP deficiency has been reported with clinical
Kyung-Soo Inn et al.
Molecular cell, 41(3), 354-365 (2011-02-05)
Upon detection of viral RNA, retinoic acid-inducible gene I (RIG-I) undergoes TRIM25-mediated K63-linked ubiquitination, leading to type I interferon (IFN) production. In this study, we demonstrate that the linear ubiquitin assembly complex (LUBAC), comprised of two RING-IBR-RING (RBR)-containing E3 ligases

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