MABC40

Anti-LAMP-2 Antibody, clone ABL-93

clone ABL-93, from rat

• Sinonimo/i: lysosomal-associated membrane protein 2, CD107 antigen-like family member B, CD107b antigen
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rat
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: ABL-93, monoclonal
• Reattività contro le specie: mouse
• tecniche: immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotipo: IgG2aκ
• N° accesso NCBI: NP_001017959
• N° accesso UniProt: P17047
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: mouse ... Lamp2(16784)

Descrizione

Descrizione generale

Lysosome-associated membrane protein 2 (LAMP-2) is a highly glycosolated protein belonging to the lysosome-associated membrane protein family. This group pf proteins composes a significant portion of the lysosomal membrane and are considered crucial mediators for cellular adhesion by interacting with carbohydrate receptors. LAMP-2 is a profuse and critical component of the lysosomal membrane as it has been implicated in playing a role in lysosomal biogenesis. It also appears to be involved in later stages of phagocytosis and autophagy, where it may act to protect the lysosomal membrane from being digested, assist in signal transduction, moderate levels of acidity within the lysosomal environment, and participate in adhesion. Mutations in LAMP-2 expression have been associated with lysosomal storage diseases or LSDs, along with Danon disease, an X-linked disorder characterized by skeletal myopathy, mental retardation, and hypertrophic cardiomyopathy.

Specificità

This antibody recognizes LAMP-2.

Immunogeno

Purified glycoproteins from NIH/3T3 cells, corresponding to mouse LAMP-2.

Applicazioni

Anti-LAMP-2 Antibody, clone ABL-93 is an antibody against LAMP-2 for use in WB, IP & IF.

Immunoprecipitation Analysis: This antibody has been shown to immunoprecipitate LAMP-2 in HaNIH cells, (Chen, 1985).

Immunofluorescence Analysis: This antibody has been shown to detect LAMP-2 in mouse brain, (Ferguson, 2009).

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Qualità

Evaluated by Western Blot in NIH/3T3 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected LAMP-2 in 10 µg of NIH/3T3 cell lysate.

Descrizione del bersaglio

~ 80 kDa observed MW. The calculated MW is 45 kDa LAMP-2 is heavily glycosoated and has been routinely observed at MW′s of 80-120 kDa based on publication data, (Chen, 1985; Ferguson, 2009).

Stato fisico

Format: Purified

Protein G

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
NIH/3T3 cell lysate

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable