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Key Documents

MABC184

Sigma-Aldrich

Anti-SDF-1 Antibody, clone K15C, Azide free

clone K15C, from mouse

Sinonimo/i:

Stromal cell-derived factor 1, SDF-1, hSDF-1, C-X-C motif chemokine 12, Intercrine reduced in hepatomas, IRH, hIRH, Pre-B cell growth-stimulating factor, PBSF

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

K15C, monoclonal

Reattività contro le specie

mouse, human

tecniche

ELISA: suitable
immunohistochemistry: suitable
neutralization: suitable
western blot: suitable

Isotipo

IgG2aκ

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... CXCL12(6387)

Descrizione generale

Stromal cell-derived factor 1 (SDF-1) is also known as C-X-C motif chemokine 12 (CXCL12), Intercrine reduced in hepatomas (IRH), and Pre-B cell growth-stimulating factor (PBSF). SDF-1 is a chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. SDF-1 stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and CXCR7, and decreases monocyte adherence to surfaces coated with ICAM-1. SDF-1 plays a protective role after myocardial infarction, has critical functions during embryonic development and is required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas.

Specificità

This antibody recognizes the N-terminus of SDF-1. This antibody should detected SDF-1alpha and SDF-1beta.

Immunogeno

BSA-conjugated linear peptide corresponding to human SDF-1.
Epitope: N-terminus

Applicazioni

Detect SDF using this mouse monoclonal antibody, Anti-SDF-1 Antibody, clone K15C, Azide free validated for use in western blotting, IHC, Neutralizing & ELISA.
Research Category
Apoptosis & Cancer
Research Sub Category
Growth Factors & Receptors
Western Blotting Analysis: 0.375 µg/mL from a representative lot detected SDF-1 in 10 µg of HeLa and RAW264.7 cell lysates.

Western Blotting Analysis: A representative lot from an independent laboratory detected SDF-1alpha, SDF-1beta, and SDF-IP10 chimera, but not IP-10, IL-8, MDC, MIP-1b, eotaxin, and RANTES in WB (Coulomb-L′Hermin, A., et al. (1999). Proc Natl Acad Sci USA. 96(15):8585-8590.).

ELISA Analysis: A representative lot from an independent laboratory detected SDF-1alpha, SDF-1beta, and SDF-IP10 chimera, but not IP-10, IL-8, MDC, MIP-1b, eotaxin, and RANTES in ELISA (Coulomb-L′Hermin, A., et al. (1999). Proc Natl Acad Sci USA. 96(15):8585-8590.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected SDF-1 in human fetal liver, lung, and kidney tissues (Coulomb-L′Hermin, A., et al. (1999). Proc Natl Acad Sci USA. 96(15):8585-8590.).

Neutralization Analysis: A representative lot from an independent laboratory attenuates the increased growth of MCF-7-ras tumor development in the presence of CAFs, decreases angiogenesis, and reduced recruitment of EPCs in tumors (Orimo, A., et al. (2005). Cell. 121(3):335-348.).

Immunhistochemistry Analysis: A representative lot from an independent laboratory detected SDF-1 in epidermal Dendritic Cells (DC) in normal and autoimmune diseased skin tissue (Pablos, J. L., et al. (1999). Am J Pathol. 155(5):1577-1586.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected SDF-1 in mucosal surfaces of small intestine tissue, rectal tissue, vaginal tissue, and endocervical tissue (Agace, W. W., et al. (2000). Curr Biol. 10(6):325-328.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected SDF-1 in mouse bone marrow sections (Dar, A., et al (2005). Nat Immunol. Epub 2005.).

Qualità

Evaluated by Western Blotting in HEPG2 cell lysate.

Western Blotting Analysis: 0.375 µg/mL of this antibody detected SDF-1 in 10 µg of HEPG2 cell lysate.

Descrizione del bersaglio

~18 kDa observed. The calculated molecular weight of the SDF-1 monomer is 9 kDa However, the SDF-1 dimer has been observed at ~18 kDa (Veldkamp, C. T., et al. (2008). Sci Signal. 1(37):ra4.).

Stato fisico

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing PBS without preservatives.

Stoccaggio e stabilità

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Risultati analitici

Control
HEPG2 cell lysate

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Ayelet Dar et al.
Nature immunology, 6(10), 1038-1046 (2005-09-20)
Regulation of the availability of chemokine SDF-1 (CXCL12) in bone marrow is still not fully understood. Here we describe a unique function for the chemokine receptor CXCR4 expressed on bone marrow endothelial cells, which efficiently internalize circulating SDF-1, resulting in
Akira Orimo et al.
Cell, 121(3), 335-348 (2005-05-11)
Fibroblasts often constitute the majority of the stromal cells within a breast carcinoma, yet the functional contributions of these cells to tumorigenesis are poorly understood. Using a coimplantation tumor xenograft model, we demonstrate that carcinoma-associated fibroblasts (CAFs) extracted from human
Hongmin Yun et al.
Communications biology, 1, 216-216 (2018-12-12)
Glaucoma is the leading cause of irreversible vision loss, and reducing elevated intraocular pressure is currently the only effective clinical treatment. The trabecular meshwork is the main resistance site for aqueous outflow that maintains intraocular pressure. In this study, we
J L Pablos et al.
The American journal of pathology, 155(5), 1577-1586 (1999-11-07)
Stromal-cell derived factor or SDF-1 is a CXC chemokine constitutively expressed by stromal bone marrow cell cultures that binds to the G-protein-coupled receptor CXCR4. SDF-1/CXCR4 represents a unique, nonpromiscuous ligand/receptor pair that plays an essential role in prenatal myelo- and
Mitra J Hooshmand et al.
Journal of immunology (Baltimore, Md. : 1950), 199(3), 1069-1085 (2017-07-09)
Inflammatory processes play a key role in pathophysiology of many neurologic diseases/trauma, but the effect of immune cells and factors on neurotransplantation strategies remains unclear. We hypothesized that cellular and humoral components of innate immunity alter fate and migration of

Articoli

The term neurodegeneration characterizes a chronic loss of neuronal structure and function leading to progressive mental impairments.

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