MAB5232
Anti-Presenilin-1 Antibody, loop, a.a. 263-378, CT, clone PS1-loop
ascites fluid, clone PS1-loop, Chemicon®
Sinonimo/i:
Anti-ACNINV3, Anti-AD3, Anti-FAD, Anti-PS1, Anti-S182
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
mouse
Forma dell’anticorpo
ascites fluid
Tipo di anticorpo
primary antibodies
Clone
PS1-loop, monoclonal
Reattività contro le specie
rat, primate, mouse, human
Produttore/marchio commerciale
Chemicon®
tecniche
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
Isotipo
IgG1
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
dry ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... PSEN1(5663)
Descrizione generale
Presenilin is a probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Presenilin requires the other members of the gamma-secretase complex to have a protease activity. It may play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. The gamma-secretase complex stimulates cell-cell adhesion through its association with the E-cadherin/catenin complex. Under conditions of apoptosis or calcium influx, it cleaves E-cadherin promoting the disassembly of the E-cadherin/catenin complex and increasing the pool of cytoplasmic beta-catenin, thus negatively regulating Wnt signaling. Futher researcher may implicate the complex in hematopoiesis. Defects in PSEN1 have been implicated in early-onset Alzheimer disease type 3 (AD3) and late-onset Alzheimer disease (LOAD).
Specificità
Presenilin-1, loop
Immunogeno
Epitope: C-terminus
Presenilin-1, loop fusion protein (amino acids 263-378), human
Applicazioni
Anti-Presenilin-1 Antibody, loop, a.a. 263-378, C-terminus, clone PS1-loop detects level of Presenilin-1 & has been published & validated for use in IC, IH(P), IP & WB.
Immunocytochemistry:
A previous lot of this antibody was used in IC.Acetone or methanol fixed cells; or lightly fixed 2.5-3% PFA (5-10′ RT).
Immunohistochemistry:
A previous lot was used on 3% paraformaldehyde fixed tissue. Works on paraffin embedded tissue sections if fixed with PLP, acetone or other light fixations; 10% formalin fixation is untested.
Immunoprecipitation:
A previous lot of this antibody was used in IP.
Western blot:
Recognizes a protein of approximately 18-20 kD, and occasionally there is a doublet (the top band of which is phosphorylated).
Optimal working dilutions must be determined by the end user
A previous lot of this antibody was used in IC.Acetone or methanol fixed cells; or lightly fixed 2.5-3% PFA (5-10′ RT).
Immunohistochemistry:
A previous lot was used on 3% paraformaldehyde fixed tissue. Works on paraffin embedded tissue sections if fixed with PLP, acetone or other light fixations; 10% formalin fixation is untested.
Immunoprecipitation:
A previous lot of this antibody was used in IP.
Western blot:
Recognizes a protein of approximately 18-20 kD, and occasionally there is a doublet (the top band of which is phosphorylated).
Optimal working dilutions must be determined by the end user
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
Qualità
Routinely evaluated by Western Blot on PC12 lysates.
Western Blot Analysis:
1:500 dilution of this lot detected PRESENILIN-1 on 10 μg of PC12 lysates.
Western Blot Analysis:
1:500 dilution of this lot detected PRESENILIN-1 on 10 μg of PC12 lysates.
Descrizione del bersaglio
18-20 kDa
Stato fisico
Mouse monoclonal IgG1 ascites with no preservatives.
Unpurified
Stoccaggio e stabilità
Stable for 6 months at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Risultati analitici
Control
Brain tissue
Brain tissue
Altre note
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Note legali
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Raccomandato
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Structure of the catalytic pore of gamma-secretase probed by the accessibility of substituted cysteines.
Sato, C; Morohashi, Y; Tomita, T; Iwatsubo, T
The Journal of Neuroscience null
Mammalian APH-1 interacts with presenilin and nicastrin and is required for intramembrane proteolysis of amyloid-beta precursor protein and Notch.
Lee, SF; Shah, S; Li, H; Yu, C; Han, W; Yu, G
The Journal of Biological Chemistry null
Presenilin-1 regulates induction of hypoxia inducible factor-1?: altered activation by a mutation associated with familial Alzheimer's disease.
De Gasperi, R; Sosa, MA; Dracheva, S; Elder, GA
Mol. Neurodegener. null
gamma-Secretase dependent production of intracellular domains is reduced in adult compared to embryonic rat brain membranes.
Fr?nberg, J; Karlstrom, H; Winblad, B; Tjernberg, LO; Frykman, S
Testing null
Mark R Farmery et al.
The Journal of biological chemistry, 278(27), 24277-24284 (2003-04-17)
One characteristic feature of Alzheimer's disease is the deposition of amyloid beta-peptide (Abeta) as amyloid plaques within specific regions of the human brain. Abeta is derived from the amyloid beta-peptide precursor protein (beta-APP) by the intramembranous cleavage activity of gamma-secretase.
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