MAB4310

Anti-CD133 Antibody, clone 13A4

clone 13A4, Chemicon^®, from rat

• Sinonimo/i: Prominin-1, AC133 (human)
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rat
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 13A4, monoclonal
• Reattività contro le specie: mouse
• Non deve reagire con: chicken, Drosophila, rat
• Produttore/marchio commerciale: Chemicon^®
• tecniche: flow cytometry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• input: sample type hematopoietic stem cell(s); sample type neural stem cell(s); sample type epithelial cells; sample type: mouse embryonic stem cell(s); sample type mesenchymal stem cell(s)
• Isotipo: IgG1κ
• Compatibilità: not suitable for immunohistochemistry (Paraffin)
• N° accesso NCBI: NM_006017.1
• N° accesso UniProt: O43490
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: dog ... Prom1(488816) ; human ... PROM1(8842) ; mouse ... Prom1(19126) ; rat ... Prom1(60357)

Descrizione

Descrizione generale

Prominin is a novel plasma membrane protein with an N-terminal extracellular domain, five transmembrane segments flanking two short cytoplasmic loops and two large glycosylated extracellular domains, and a cytoplasmic C-terminal domain. DNA sequences from Caenorhabditis elegans predict the existence of a protein with the same features, suggesting that prominin is conserved between vertebrates and invertebrates. Prominin is found not only in the neuroepithelium but also in various other epithelia of the mouse embryo. In the adult mouse, prominin has been detected in the brain ependymal layer, and in kidney tubules. In these epithelia, prominin is specific to the apical surface, where it is selectively associated with microvilli and microvilli-related structures. Remarkably, upon expression in CHO cells, prominin is preferentially localized to plasma membrane protrusions such as filopodia, lamellipodia, and microspikes. These observations imply that prominin contains information to be targeted to, and/or retained in, plasma membrane protrusions rather than the planar cell surface. Moreover, results show that the mechanisms underlying targeting of membrane proteins to microvilli of epithelial cells and to plasma membrane protrusions of non-epithelial cells are highly related. {Weigmann, A et al.1997}. Also see {http://www.ncbi.nlm.nih.gov/prow/guide/438375806_g.htm} for more information on CD133 and prominin expression.

Specificità

Recognizes mouse Prominin-1 (sometimes also referred to as CD133 and, in the case of the human orthologue, as AC133), a 115-120 kDa pentaspan transmembrane (5-TM) domain glycoprotein. Prominin-1 is expressed on primitive cells such as hematopoietic stem and progenitor cells, neural & endothelial stem cells, retina and retinoblastoma, as well as developing epithelium. To date, the function and ligand of Prominin-1 are unknown.

This antibody can be used to isolate murine stem cells from brain and bone marrow.

The 13A4 antibody does not cross react with rat, human, chicken, or Drosophila antigen. The original paper is located at: http://www.pnas.org/cgi/reprint/94/23/12425.pdf

Immunogeno

Telencephali of ten 12-day-old NMRI mouse embryos (E12, ~1 mg protein), which consist mostly of neuroepithelium, were homogenized in 1 ml PBS, mixed with crushed nitrocellulose filter (1 cm2 in 0.5 ml PBS) as adjuvant, and injected i.p. into a LouXSD rat. {Weigmann et al, PNAS 1997}

Applicazioni

Anti-CD133 Antibody, clone 13A4 detects level of CD133 & has been published & validated for use in FC, IH, IP & WB.

Western Blot Analysis: A 1:1000 dilution from a representative lot detected CD133 in monkey kidney lysate.

Research Category
Stem Cell Research

Research Sub Category
Neural Stem Cells

Hematopoietic Stem Cells

Descrizione del bersaglio

115 kDa

Stato fisico

Format: Purified

Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.

Protein A purified

Stoccaggio e stabilità

Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Risultati analitici

Control
Positive Control: Kidney tissue or membranes
Negative Control: Liver tissue or membranes

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable