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Documenti fondamentali

MAB3507

Sigma-Aldrich

Anti-Caspase 2 Antibody, clone 11B4

clone 11B4, Chemicon®, from rat

Sinonimo/i:

ICH-1

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

rat

Livello qualitativo

Forma dell’anticorpo

purified antibody

Tipo di anticorpo

primary antibodies

Clone

11B4, monoclonal

Reattività contro le specie

mouse, monkey, human, canine

Produttore/marchio commerciale

Chemicon®

tecniche

ELISA: suitable
flow cytometry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG2a

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... CASP2(835)

Specificità

MAB3507 antibody recognizes a reactive epitope that lies in the P19 subunit of human Caspase-2 (ICH-1, Nedd-2).

Immunogeno

Raised against the P19 subunit of recombinant human Caspase-2 protein.

Applicazioni

Anti-Caspase 2 Antibody, clone 11B4 is an antibody against Caspase 2 for use in ELISA, FC, IP & WB.
Research Category
Apoptosis & Cancer

Metabolism
Research Sub Category
Caspases

Enzymes & Biochemistry
Western blot: Recommended usage at 5 μg/ml, which has been found to detect endogenous Caspase-2 in 5 x 10E5 HeLa and MCF-7 cells.

Immunoprecipitation: Recommended concentration 1-5 μg/ml FACS: Recommended concentration 1 μg/ml. It is necessary to permeabilize the cell membrane prior to analysis.

EIA: Recommended concentration 0.5-1 μg/ml.

Immunohistochemistry: Use at 10-20 μg/ml. It is necessary to fix the cells in 4% Paraformaldehyde/0.18% Triton-X100 for 10 minutes prior to staining.

Optimal working dilutions must be determined by end user.

Linkage

Replaces: 04-571

Stato fisico

Format: Purified
Liquid with 0.05% Bronidox as a preservative.

Stoccaggio e stabilità

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Intrinsic caspase-8 activation mediates sensitization of erlotinib-resistant tumor cells to erlotinib/cell-cycle inhibitors combination treatment.
Orzaez, M; Guevara, T; Sancho, M; Perez-Paya, E
Cell Death & Disease null
Squamous cell carcinoma antigen 1 promotes caspase-8-mediated apoptosis in response to endoplasmic reticulum stress while inhibiting necrosis induced by lysosomal injury.
Ullman, E; Pan, JA; Zong, WX
Molecular and cellular biology null
Christopher J Del Nagro et al.
Cell cycle (Georgetown, Tex.), 13(2), 303-314 (2013-11-20)
Activation of Checkpoint kinase 1 (Chk1) following DNA damage mediates cell cycle arrest to prevent cells with damaged DNA from entering mitosis. Here we provide a high-resolution analysis of cells as they undergo S- and G₂-checkpoint bypass in response to
Brittany L Carroll et al.
FEBS open bio, 8(1), 27-40 (2018-01-13)
Sphingosine kinase 1 (SK1) is a lipid kinase whose activity produces sphingosine 1-phosphate, a prosurvival lipid associated with proliferation, angiogenesis, and invasion. SK1 overexpression has been observed in numerous cancers. Recent studies have demonstrated that SK1 proteolysis occurs downstream of
M Mancini et al.
The Journal of cell biology, 149(3), 603-612 (2000-05-03)
Caspases are an extended family of cysteine proteases that play critical roles in apoptosis. Animals deficient in caspases-2 or -3, which share very similar tetrapeptide cleavage specificities, exhibit very different phenotypes, suggesting that the unique features of individual caspases may

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