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MAB3484

Sigma-Aldrich

Anti-Cystic Fibrosis Transmembrane Conductance Regulator Antibody, a.a. 386-412, clone L12B4

clone L12B4, Chemicon®, from mouse

Sinonimo/i:

CFTR

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

100

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

L12B4, monoclonal

Reattività contro le specie

human

Non deve reagire con

shark, mouse

Produttore/marchio commerciale

Chemicon®

tecniche

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG2a

N° accesso NCBI

NM_000492.3

N° accesso UniProt

P13569

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... CFTR(1080)

Specificità

Human cystic fibrosis transmembrane conductance regulator (CFTR). Recognizes an epitope at the cytoplasmic region preceding the first nucleotide binding domain between residues 386 to 412.

Recognizes CFTR, Mr 170kDa and two additional proteins at ~ Mr 100 and 140kDa.

Immunogeno

Epitope: a.a. 386-412 of human CFTR

Applicazioni

Anti-Cystic Fibrosis Transmembrane Conductance Regulator Antibody, a.a. 386-412, clone L12B4 is an antibody against Cystic Fibrosis Transmembrane Conductance Regulator for use in IC, IP & WB.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
Western blot: 1-10μg/mL, note do not boil the lysate, incubate at 80C for 30 minutes prior to running SDS-PAGE. CTFR aggregates upon boiling. Antibody recognizes CFTR at 170kDa and two additional proteins at 100 & 140kDa.

Immunoprecipitation

Immunofluorescence

Note: Does not work on paraffin embedded tissue.

Optimal working dilutions must be determined by the end user.

Stato fisico

Format: Purified
Purified immunoglobulin. Liquid in 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.

Stoccaggio e stabilità

Maintain at 2-8°C in undiluted aliquots up to 6 months.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Targeted quantitation of overexpressed and endogenous cystic fibrosis transmembrane conductance regulator using multiple reaction monitoring tandem mass spectrometry and oxygen stable isotope dilution.
Jiang H, Ramos AA, Yao X
Analytical Chemistry null
Lacking CD56 expression in a relapsing cutaneous blastic plasmacytoid dendritic cell neoplasm after allogeneic bone marrow transplantation: FISH analysis revealed loss of 11q.
Mitteldorf C, Bertsch HP, Baumgart M, Haase D, Wulf G, Schon MP, Rosenwald A, Neumann C, Kaune KM.
Journal of the European Academy of Dermatology and Venereology : JEADV null
Cysteine string protein interacts with and modulates the maturation of the cystic fibrosis transmembrane conductance regulator.
Zhang, H; Peters, KW; Sun, F; Marino, CR; Lang, J; Burgoyne, RD; Frizzell, RA
The Journal of Biological Chemistry null
Mauricio Di Fulvio et al.
PloS one, 15(12), e0242749-e0242749 (2020-12-03)
Cystic fibrosis (CF) is due to mutations in the CF-transmembrane conductance regulator (CFTR) and CF-related diabetes (CFRD) is its most common co-morbidity, affecting ~50% of all CF patients, significantly influencing pulmonary function and longevity. Yet, the complex pathogenesis of CFRD
Ana Isabel Mendes et al.
Molecular and cellular biology, 31(19), 4076-4086 (2011-08-03)
Members of the WNK (with-no-lysine [K]) subfamily of protein kinases regulate various ion channels involved in sodium, potassium, and chloride homeostasis by either inducing their phosphorylation or regulating the number of channel proteins expressed at the cell surface. Here, we
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