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MAB3199Z

Sigma-Aldrich

Anti-E-Cadherin Antibody, clone 67A4, Azide Free

clone 67A4, Chemicon®, from mouse

Sinonimo/i:

CD324, MAB3199

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

100

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

67A4, monoclonal

Reattività contro le specie

human

Confezionamento

antibody small pack of 25 μg

Produttore/marchio commerciale

Chemicon®

tecniche

flow cytometry: suitable
immunofluorescence: suitable

Isotipo

IgG1

N° accesso NCBI

NM_004360.2

N° accesso UniProt

P12830

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... CDH1(999)

Categorie correlate

Specificità

Antibodies against E-cadherin are suitable to characterize epithelial cells. E-Cadherin is involved in adhesion of epithelial cells (mostly homotypic and homophilic). E-cadherin is also expressed on immature erythroid cells. The antibody 67A4 is suitable to study E cadherin expression by flow cytometry. In addition, it blocks the interaction of E-cadherin molecules and therefore is suitable to study E-cadherin-mediated signal transduction.

Immunogeno

T-47D breast carcinoma line.

Applicazioni

Anti-E-Cadherin Antibody, clone 67A4, Azide Free is an antibody against E-Cadherin for use in FC & IF.
Immunoblotting

FACS Analysis

Functional blocking of E-cadherin

Does not work well for immunoprecipitation

Optimal working dilutions must be determined by end user.

Qualità

Tested

Stato fisico

Format: Purified
Liquid in 0.02M PBS with NaCl, pH=7.6, with no preservatives. Protein A purified immunoglobulin.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Certificati d'analisi (COA)

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Journal of cell science, 115(Pt 23), 4505-4515 (2002-11-05)
Cadherins are a family of cell adhesion molecules that mainly mediate homotypic homophilic interactions, but for E-cadherin, heterophilic interactions with the integrin alpha(E)(CD103)beta(7) have also been reported. In the human thymus, where thymocytes develop in close contact with thymic stromal
The adhesion molecule E-cadherin and a surface antigen recognized by the antibody 9C4 are selectively expressed on erythroid cells of defined maturational stages.
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