MAB1922
Anti-Laminin α2 Antibody, clone 5H2
ascites fluid, clone 5H2, Chemicon®
Sinonimo/i:
Laminin M chain, Merosin heavy chain, laminin M, laminin alpha 2 subunit, laminin, alpha 2
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Forma dell’anticorpo
ascites fluid
Tipo di anticorpo
primary antibodies
Clone
5H2, monoclonal
Reattività contro le specie
human, rabbit, monkey
Produttore/marchio commerciale
Chemicon®
tecniche
ELISA: suitable
cell culture | mammalian: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotipo
IgG1
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
dry ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... LAMA2(3908)
rhesus monkey ... Lama2(715394)
Descrizione generale
Elements of the extracellular matrix such as laminins, a family of heterotrimeric extracellular glycoproteins, affect tissue development and integrity in organs including the kidney, lung, skin, and nervous system. Laminins function as heterotrimeric complexes of alpha, beta, and gamma chains, with each chain type representing a different subfamily of proteins. For example, the alpha subfamily of laminin chains is a major component of basement membranes. Two transcript variants encoding different isoforms have been found for this gene, but the full-length nature of one of them has not been determined. At least 15 distinct laminin trimers, containing various combinations of 5 alpha, 4 beta, and 3 gamma subunits have been found in mammals. Both laminin alpha 5 and alpha 1 are prevalent in kidney. Mice that are homozygous for a null mutation in the alpha 5 laminin gene are dead by embrionic day 14 - 19 with multiple developmental abnormalities. The kidney phenotypes include avascular glomeruli, impaired branching morphogenesis, and renal agenesis.
Specificità
Cross reacts with monkey and rabbit merosin.
Reacts with the 80 kDa fragment of the M-chain of human merosin.
Immunogeno
Purified human merosin
Applicazioni
Detect Laminin α2 using this Anti-Laminin α2 Antibody, clone 5H2 validated for use in ELISA, CULT, IF, IH, IP & WB.
ELISA:
50% maximal binding to human merosin at 1:50,000 dilution from a previous lot.
Immunohistochemistry:
1:5,000 dilution from a previous lot was used for staining of 8 µm acetone-fixed cryostat muscle sections, prior to detection with a peroxidase-conjugated secondary antibody.
Immunofluorescence:
A previous lot of this antibody was used in immunofluorescent.
Affinity chromatography:
A previous lot of this antibody was used in IAP.
Immunoprecipitation:
A previous lot of this antibody was used in IP.
Optimal working dilutions must be determined by end user.
50% maximal binding to human merosin at 1:50,000 dilution from a previous lot.
Immunohistochemistry:
1:5,000 dilution from a previous lot was used for staining of 8 µm acetone-fixed cryostat muscle sections, prior to detection with a peroxidase-conjugated secondary antibody.
Immunofluorescence:
A previous lot of this antibody was used in immunofluorescent.
Affinity chromatography:
A previous lot of this antibody was used in IAP.
Immunoprecipitation:
A previous lot of this antibody was used in IP.
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
Qualità
Routinely evaluated by Western Blot on Human Placenta lysate.
Western Blot Analysis:
1:1000 dilution of this lot detected Laminin α2 (merosin) on 10 μg of Human Placenta lysate.
Western Blot Analysis:
1:1000 dilution of this lot detected Laminin α2 (merosin) on 10 μg of Human Placenta lysate.
Descrizione del bersaglio
80 kDa
Stato fisico
Unpurified
Unpurified ascites in buffer containing no preservatives.
Stoccaggio e stabilità
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage the IgG1 and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage the IgG1 and affect product performance.
Risultati analitici
Control
Vascular breast carcinomas, Cultured embryonic retinal neurons and RGCs
Vascular breast carcinomas, Cultured embryonic retinal neurons and RGCs
Altre note
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Note legali
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Raccomandato
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Holly Colognato et al.
Development (Cambridge, England), 134(9), 1723-1736 (2007-03-31)
Developmental abnormalities of myelination are observed in the brains of laminin-deficient humans and mice. The mechanisms by which these defects occur remain unknown. It has been proposed that, given their central role in mediating extracellular matrix (ECM) interactions, integrin receptors
The expanding phenotype of laminin alpha2 chain (merosin) abnormalities: case series and review.
K J Jones, G Morgan, H Johnston, V Tobias, R A Ouvrier, I Wilkinson, K N North
Journal of medical Genetics null
Calpain 3 gene mutations: genetic and clinico-pathologic findings in limb-girdle muscular dystrophy.
J Chae, N Minami, Y Jin, M Nakagawa, K Murayama, F Igarashi, I Nonaka
Neuromuscular Disorders null
Merosin, a protein specific for basement membranes of Schwann cells, striated muscle, and trophoblast, is expressed late in nerve and muscle development.
Leivo, I and Engvall, E
Proceedings of the National Academy of Sciences of the USA, 85, 1544-1548 (1988)
Disruption of muscle basal lamina in congenital muscular dystrophy with merosin deficiency.
C Minetti, M Bado, G Morreale, M Pedemonte, G Cordone
Neurology null
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