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Key Documents

MAB1904

Sigma-Aldrich

Anti-Laminin-1 A&B chains Antibody, cross region, clone AL-2

clone AL-2, Chemicon®, from rat

Sinonimo/i:

laminin alpha1, laminin beta1

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

rat

Livello qualitativo

Forma dell’anticorpo

purified antibody

Tipo di anticorpo

primary antibodies

Clone

AL-2, monoclonal

Reattività contro le specie

mouse

Produttore/marchio commerciale

Chemicon®

tecniche

ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG1κ

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

mouse ... Lama1(16772)

Descrizione generale

Laminins are secreted glycoproteins incorporated into cell-associated extracellular matrices and an integral part of the structural scaffolding in most animal tissues. The laminin protein is made up of three polypeptide chains; α, β and γ, accounting for its flexibility in connecting various molecules. When originally characterized, laminin-1 clone AL-2 reacted with Engelbreth-Holm-Swarm (EHS) mouse laminin A & B chains on the long arm near the cross region, (Skubitz, 1987). More recently, investigations, (Schèele, 2006) have shown that the clone binds laminin β1 under reducing conditions as well as native ELISAs and that the affinity is increased by the presence of the laminin α1 chain. Thus there is significant laminin β1 reactivity with clone AL-2.

Specificità

Other species have not been tested.
Reacts with EHS mouse laminin A & B chains on the long arm near the cross region.

Immunogeno

Epitope: Near cross region
Laminin isolated from EHS mouse sarcoma.

Applicazioni

Detect Laminin-1 A&B chains using this Anti-Laminin-1 A&B chains Antibody, cross region, clone AL-2 validated for use in ELISA, IF, IH(P), IP & WB.
Immunofluorescence:
An independent laboratory (Schèele, S., 2006) has verified anti-laminin clone AL-2 to work using immunofluorescence techniques on mouse muscle tissue, including both transgenic and wild-type tissues.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

Qualità

Evaluated on a representative lot by Western blot on purified mouse laminin-1.

Descrizione del bersaglio

~200 kDa

Stato fisico

Format: Purified
Protein G Purified
Purified in 0.1M Tris-Glycine (pH 7.4) 150mM NaCl with 0.05% NaN3.

Stoccaggio e stabilità

Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Laminin isoforms 8 and 10 are primary components of the subendothelial basement membrane promoting interaction with neoplastic lymphocytes.
P Spessotto, Z Yin, G Magro, R Deutzmann, A Chiu, A Colombatti, R Perris
Cancer Research null
Clinical and histopathological analysis of healing process of intraoral reconstruction with ex vivo produced oral mucosa equivalent.
Takeshi Hotta,Satoshi Yokoo,Hiroto Terashi,Takahide Komori
Kobe J. Med. Sci. null
George S Karagiannis et al.
Molecular oncology, 8(2), 178-195 (2013-11-26)
It has been suggested that cancer-associated fibroblasts (CAFs) positioned at the desmoplastic areas of various types of cancer are capable of executing a migratory program, characterized by accelerated motility and collective configuration. Since CAFs are reprogrammed derivatives of normal progenitors
Localization of three distinct heparin-binding domains of laminin by monoclonal antibodies.
Skubitz, A P, et al.
The Journal of Biological Chemistry, 263, 4861-4868 (1988)
Susanne Schéele et al.
Matrix biology : journal of the International Society for Matrix Biology, 25(5), 301-305 (2006-04-25)
We analyzed the reactivity of three different commercially available rat monoclonal antibodies raised against mouse laminin-alpha1beta1gamma1 (laminin-111), AL-1, AL-2, and AL-4. Using ELISA assays, Western blot analysis and immunostainings we present refined epitope maps for these three laminin monoclonals. AL-1

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