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Key Documents

MAB1379

Sigma-Aldrich

Anti-CD54 Antibody, extracellular, clone 84H10

clone 84H10, Chemicon®, from mouse

Sinonimo/i:

ICAM-1

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

affinity purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

84H10, monoclonal

Reattività contro le specie

human

Non deve reagire con

canine

Produttore/marchio commerciale

Chemicon®

tecniche

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

Isotipo

IgG1

Compatibilità

not suitable for immunohistochemistry (Paraffin)

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... ICAM1(3383)

Descrizione generale

This molecule is inducible to high levels by mitogenic lectins on lymphocytes and by IL-1beta or IFN gamma on other cell types such as fibroblasts and enthothelial cells.

Specificità

Recognizes the extracellular D1 domain of ICAM-1. Blocks binding of LFA-1 and P. falciparum to ICAM-1. Reacts with the ICAM-1 antigen found in low levels on lymphocytes and strongly expressed on monocytes and granulocytes. Detects an antigen of 90 kDa.

Immunogeno

Epitope: extracellular
K562 cell line

Applicazioni

Detect CD54 using this Anti-CD54 Antibody, extracellular, clone 84H10 validated for use in FC, IP, IH.
Flow Cytometry: Neat - 1:10. 10 μL of suggested dilution will label 10e6 cells.

Immunohistochemistry on frozen tissue sections: 1:20-1:50. Does not work on paraffin embedded tissue sections.

Immunoprecipitation: 5 μg/ 10e7 cells

Adhesion Blocking; blocks ICAM-1 mediated adhesion to LFA-1.

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)

Stato fisico

Affinity purified immunoglobulin. Liquid in Phosphate buffered saline, pH 7.4, containing 0.09% sodium azide.
Format: Purified

Stoccaggio e stabilità

Store at 2-8°C for up to 12 months from date of receipt. Alternatively, this antibody can be stored at -20ºC. Avoid repeated freeze-thaw cycles.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Li Wang et al.
Haematologica, 96(6), 927-931 (2011-02-19)
Vascular endothelial growth factor-A, an angiogenesis stimulator expressed on both tumor endothelial and malignant T cells, is involved in tumor progression in T-leukemia/lymphoma. Here, we assessed the impact of therapeutic vascular endothelial growth factor-A blockade on tumor-endothelial cell interaction and
Flow-conditioned HUVECs support clustered leukocyte adhesion by coexpressing ICAM-1 and E-selectin.
Burns, MP; DePaola, N
American Journal of Physiology. Heart and Circulatory Physiology null
Timo Rath et al.
Scandinavian journal of gastroenterology, 45(10), 1186-1196 (2010-06-24)
Matrix metalloproteinases (MMPs) are considered the predominant proteases in the pathogenesis of mucosal ulcerations associated with inflammatory bowel disease (IBD). Whether the malignancy associated MMP-7 and MMP-13 or the recently cloned MMP-28 convey a certain meaning for intestinal homeostasis and
Synergistic induction of intercellular adhesion molecule-1 by the human cytomegalovirus transactivators IE2p86 and pp71 is mediated via an Sp1-binding site.
Martina Kronschnabl, Thomas Stamminger
The Journal of General Virology null

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