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Key Documents

HTS089M

Sigma-Aldrich

ChemiSCREEN Membrane Preparation Recombinant Human LPA1 Lysophospholipid Receptor

Human LPA1 / EDG2 GPCR membrane preparation for GTPγS binding Assays.

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About This Item

Codice UNSPSC:
41106514
eCl@ss:
32161000
NACRES:
NA.84

Origine biologica

human

Livello qualitativo

Ricombinante

expressed in Chem-1 cells

Produttore/marchio commerciale

ChemiScreen
Chemicon®

tecniche

ligand binding assay: suitable (GTPγS)

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

dry ice

Descrizione generale

Human EDG2 encoding LPA1
Lysophosphatidic acid (LPA) is a lysophospholipid produced by activated platelets that inhibits adenylate cyclase and stimulates DNA synthesis, changes in cell morphology, and increases in intracellular calcium in a variety of cultured mammalian cells. A family of three GPCRs, LPA1, LPA2 and LPA3, mediates the biological effects of LPA (Contos et al., 2000). LPA1 is widely expressed, with particularly notable expression in the ventricular zone of the embryonic cerebral cortex. Mice lacking LPA1 exhibit craniofacial abnormalities and defective development of the cerebral cortex (Harrison et al., 2003; Choi et al., 2008). LPA1 also promotes fibrotic responses to tissue injury (Tager et al., 2008)

Millipore′s LPA1 Membrane Preparation is a crude membrane preparation made from the proprietary stable recombinant cell line that is also used for Millipore’s LPA1 Calcium Optimized Cell Line (HTS089C). This LPA1 Membrane Preparation exhibits both constitutive and agonist-induced activity in GTPγS binding assays; thus it is an ideal HTS tool for screening of compounds for agonist, inverse agonist, and antagonist activities. The membrane preparations exhibits EC50 of 73 nM for LPA in a GTPγS binding assay.

Applicazioni

GTPγS binding assay

Azioni biochim/fisiol

GPCR Class: A
Protein Target: LPA1 / EDG2
Target Sub-Family: Lysophospholipid

Qualità

1 unit = 5 μg
EC50 in GTPγS binding assay by LPA: ~ 73 nM

Specifiche

Inucbation Conditions
GTPγS ASSAY CONDITIONS: Membranes are permeabilized by addition of saponin to an equal concentration by mass, then mixed with [35S]-GTPγS (final concentration of 0.3 nM) in 20 mM HEPES, pH 7.4/100 mM NaCl/10 mM MgCl2/10 μM GDP in a nonbinding 96-well plate. Unlabeled ligand was added to the final concentration indicated in Figure 1 (final volume 100 μL), and incubated for 30 min at 30°C. The binding reaction is transferred to an FB filter plate (Millipore MAHF B1H) previously prewetted with water. The plate is washed 3 times (1 mL per well per wash) with cold 10 mM sodium phosphate, pH 7.4, then dried and counted.

Stato fisico

One package contains enough membranes for at least 200 assays (units), where one unit is the amount of membrane that will yield greater than 1000 cpm specific LPA-stimulated [35S]-GTPγS binding.
Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membrane protein was adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.

Stoccaggio e stabilità

Store at –70°C. Product is stable for at least 6 months from the date of receipt when stored as directed. Do not freeze and thaw.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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