GR13L

Anti-EGFR (Ab-3) Mouse mAb (225)

lyophilized, clone 225, Calbiochem^®

• Sinonimo/i: Anti-EGF-R
• Codice UNSPSC: 12352203
• NACRES: NA.43

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 225, monoclonal
• Stato: lyophilized
• non contiene: preservative
• Reattività contro le specie: human
• Non deve reagire con: rat, mouse
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze
• Isotipo: IgG1
• Condizioni di spedizione: ambient
• Temperatura di conservazione: 2-8°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... EGFR(1956) ; mouse ... Egfr(13649) ; rat ... Egf(25313)

Descrizione

Descrizione generale

Anti-EGFR (Ab-3), mouse monoclonal, clone 225 recognizes the ~170 kDa epidermal growth factor receptor protein. It is validated for use in immunoprecipitation and neutralization.

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~170 kDa epidermal growth factor receptor protein.

Recognizes the ~170 kDa epidermal growth factor receptor protein in A431 cells.

Immunogeno

Human

partially purified, human EGFR from A431 cells

Applicazioni

Immunoprecipitation (1-2 µg per sample)

Neutralization Studies (2 µg/ml, see comments and application references)

Attenzione

Toxicity: Standard Handling (A)

Stato fisico

Lyophilized from 20 mM ammonium bicarbonate solution, 100 µg BSA.

Ricostituzione

Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate at 4°C with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.

Risultati analitici

Negative Control
HL-60 cells

Positive Control
A431 cells

Altre note

Hunter T. 1984. Nature311, 414.
Reynolds, F.H., et. al 1981. Nature292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.

This antibody has been shown to inhibit human tumor xenographs in nude mice. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. It also inhibits A431 tumor formation in nude mice. Antibody should be titrated for optimal results in individual systems.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): nwg
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable