GF091

Soluble RANK Ligand (sRANKL) Protein, Recombinant human

The recombinant human sRANKL protein is produced in E. Coli.

• Sinonimo/i: sRANKL, Soluble Receptor Activator of NF-Kappa B, TNFSF11, OPGL, ODF
• Codice UNSPSC: 12352202
• eCl@ss: 32160405

Proprietà

• Origine biologica: human
• Livello qualitativo: 100
• Ricombinante: expressed in E. coli
• Saggio: >98% (SDS-PAGE and HPLC)
• Produttore/marchio commerciale: Chemicon^®
• tecniche: cell culture | mammalian: suitable
• Impurezze: <0.1 ng/μg Endotoxin (1EU/μg)
• input: sample type mesenchymal stem cell(s)
• N° accesso NCBI: NM_003701.2; NM_033012.2
• N° accesso UniProt: O14788
• Condizioni di spedizione: dry ice
• Informazioni sul gene: human ... TNFSF11(8600)

Descrizione

Descrizione generale

Product Source: E. coli

RANKL and RANK are members of the TNF superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone turnover. RANK (receptor) was originally identified as a dendritic-cell-membrane protein, which by interacting with RANKL augments the ability of dendritic cells to stimulate naïve T-cell proliferation in a mixed lymphocyte reaction, to promote the survival of RANK + T cells, and to regulate T-cell-dependent immune response. RANKL, which is expressed in a variety of cells including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoy receptor called OPG. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage.

Recombinant human sRANKL produced in E. coli. The protein is a 20.0 kDa polypeptide comprising the TNF homologous region of RANKL (176 amino acid residues).SEQUENCE: MEKAMVDGSW LDLAKRSKLE AQPFAHLTIN ATDIPSGSHK VSLSSWYHDR GWAKISNMTF SNGKLIVNQD GFYYLYANIC FRHHETSGDL ATEYLQLMVY VTKTSIKIPS SHTLMKGGST KYWSGNSEFH FYSINVGGFF KLRSGEEISI EVSNPSLLDP DQDATYFGAF KVRDID

COUNTRY OF ORIGIN: USA

Stato fisico

Sterile filtered, and then lyophilized from 5 mM sodium phosphate, pH 7.6 + 75 mM NaCl. Reconstitute with distilled water to a concentration of 0.1-1 mg/mL as a stock solution. Solution can then be diluted into other aqueous buffers for immediate use.

Stoccaggio e stabilità

The lyophilized powder is stable for several weeks at room temperature, but best stored at -20°C for up to one year from date of receipt. Reconstituted stock material can be stored at 2°-8°C for one week, but undiluted aliquots at -20°C are recommended for longer use. Avoid repeated freeze/thaw cycles.

Risultati analitici

Specific Activity: Determined by its ability to induce osteoclast formation in RAW264.7 cells using a concentration range of 5.0-10.0 ng/mL.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1