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Key Documents

CC43

Sigma-Aldrich

Anti-Cdh1 (Ab-2) Mouse mAb (DH01)

liquid, clone DH01, Calbiochem®

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.43

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

purified antibody

Tipo di anticorpo

primary antibodies

Clone

DH01, monoclonal

Forma fisica

liquid

contiene

≤0.1% sodium azide as preservative

Reattività contro le specie

human

Produttore/marchio commerciale

Calbiochem®

Condizioni di stoccaggio

do not freeze

Isotipo

IgG1

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... CDH1(999)

Descrizione generale

Anti-Cdh1 (Ab-2), mouse monoclonal, clone DH01, recognizes the ~55 kDa Cdh-1 protein in Rat-1 cells. It is validated for Western blotting and immunoprecipitation.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes the ~55 kDA Cdh1 protein.
Recognizes the ~55 kDa Cdh-1 protein in Rat-1 cells.

Immunogeno

Human
recombinant human Cdh1 protein

Applicazioni


Immunoblotting (1 g/ml)
Immunoprecipitation (2 g/mg protein lysate)

Confezionamento

Please refer to vial label for lot-specific concentration.

Attenzione

Toxicity: Standard Handling (A)

Stato fisico

In 10 mM PBS, 0.2% BSA, pH 7.4.

Risultati analitici

Negative Control
Normal mouse serum
Positive Control
Junkat or LS174T cells

Altre note

Does not cross-react with other WD repeat containing proteins, including Cdc20. Antibody should be titrated for optimal results in individual systems.
Kramer, E.R., et al. 2000 Mol. Biol. Cell11, 1555.
Petersen, B.O., et al. 2000 Genes Dev.14, 2330.
Pfleger, C.M., et al. 2001 Genes Dev.15, 1759.
Sorensen, C.S., et al. 2001 Mol. Cell Biol.21, 3692.
Gieffers, C., et al. 1999 Proc. Natl. Acad. Sci. USA96, 11317.
Visintin, R., et al. 1997 Science278, 460.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Hidefumi Fukushima et al.
Cell reports, 1(5), 434-443 (2012-06-19)
The NFkB/Rel family of proteins play critical roles in a variety of cellular processes. Thus, their physiological activation is tightly controlled. Recently, the NFkB2/p100 precursor has been characterized as the fourth IkB type of suppressor for NFkB. However, the molecular
Dipankar Ray et al.
Molecular and cellular biology, 25(8), 3338-3347 (2005-03-31)
Ubiquitin-dependent degradation of Cdc25A is a major mechanism for damage-induced S-phase checkpoint. Two ubiquitin ligases, the Skp1-cullin-beta-TrCP (SCFbeta-TrCP) complex and the anaphase-promoting complex (APCCdh1), are involved in Cdc25A degradation. Here we demonstrate that the transforming growth factor beta (TGF-beta)-Smad3 pathway
Jia Liu et al.
Cell discovery, 2, 15044-15044 (2016-07-28)
Anaphase-promoting complex/cyclosome/Cdh1 is a multi-subunit ubiquitin E3 ligase that drives M to G1 cell cycle progression through primarily earmarking various substrates for ubiquitination and subsequent degradation by the 26S proteasome. Notably, emerging evidence suggested that Cdh1 could also function in
Kyung Uk Hong et al.
The Journal of biological chemistry, 284(24), 16501-16512 (2009-04-17)
During mitosis, establishment of structurally and functionally sound bipolar spindles is necessary for maintaining the fidelity of chromosome segregation. Tumor-associated microtubule-associated protein (TMAP), also known as cytoskeleton-associated protein 2 (CKAP2), is a mitotic spindle-associated protein whose level is frequently up-regulated
Savvas C Pavlides et al.
Cell cycle (Georgetown, Tex.), 15(7), 931-947 (2016-03-11)
We previously reported that aberrant TGF-β/Smad2/3 signaling in endometrial cancer (ECA) leads to continuous ubiquitylation of p27(kip1)(p27) by the E3 ligase SCF-Skp2/Cks1 causing its degradation, as a putative mechanism involved in the pathogenesis of this cancer. In contrast, normal intact

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