CBL171
Anti-Actin Antibody, smooth muscle, clone ASM-1
clone ASM-1, Chemicon®, from mouse
Sinonimo/i:
Alpha-actin-2
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
ASM-1, monoclonal
Reattività contro le specie
chicken, mouse, horse, rat, human, bovine
Produttore/marchio commerciale
Chemicon®
tecniche
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
Isotipo
IgG2a
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
dry ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... ACTA2(59)
Descrizione generale
ASM-1 represents an excellent marker for myogenic soft tissue tumors and smooth muscle differentiation. This antibody reacts with many types of smooth muscle cells, such as those present in vascular walls, intestinal muscularis mucosae and propria, myometrium, stroma of various tissues, and is also positive for myoepithelial cells of various glands, notably salivary and mammary gland. Myogenic soft tissues detected include leiomyosarcomas, leiomyomas, and certain stromal cells surrounding infiltrating ductal carcinoma of the breast.
Specificità
Expected to cross-react with bovine, equine and chicken. Reactivity with other species has not been confirmed.
This antibody is specific for the alpha-smooth-muscle isoform of actin (MW 43 kDa).
Immunogeno
Epitope: N-terminus
Synthetic peptide corresponding to the ten N-terminal amino acids of the alpha-smooth muscle isoform of actin.
Applicazioni
Anti-Actin Antibody, smooth muscle, clone ASM-1 detects level of Actin & has been published & validated for use in IF, IH, IH(P) & WB.
Immunohistochemistry:
1:2000 dilution of a previous lot was used on frozen and formalin-fixed, paraffin-embedded tissues; protease pretreatment is recommended for paraffin-embedded sections.
Immunofluorescence:
A previous lot of this antibody was used on Immunofluoresence.
Optimal working dilutions must be determined by the end user.
1:2000 dilution of a previous lot was used on frozen and formalin-fixed, paraffin-embedded tissues; protease pretreatment is recommended for paraffin-embedded sections.
Immunofluorescence:
A previous lot of this antibody was used on Immunofluoresence.
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
Qualità
Evaluated by Western Blot on HUVEC lysates.
Western Blot Analysis:
1:1000 dilution of this antibody detected ACTIN, SM MUSC on 10 μg of HUVEC lysates.
Western Blot Analysis:
1:1000 dilution of this antibody detected ACTIN, SM MUSC on 10 μg of HUVEC lysates.
Descrizione del bersaglio
43 kDa
Linkage
Replaces: 04-1040
Stato fisico
Format: Purified
Protein A purified
Purified mouse monoclonal IgG2a presented as lyophilized material. Reconstitute in 1 mL of of distilled water.
Final solution is PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide.
Final solution is PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide.
Stoccaggio e stabilità
Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Risultati analitici
Control
Positive reaction using stress fibers of smooth muscle-derived cells and some smooth muscle subtype fibroblasts.
Positive reaction using stress fibers of smooth muscle-derived cells and some smooth muscle subtype fibroblasts.
Note legali
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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