ABS79

Anti-phospho-mTOR (Ser2159) Antibody

from rabbit, purified by affinity chromatography

• Sinonimo/i: Serine/threonine-protein kinase mTOR, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, Mammalian target of rapamycin, mTOR, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapam
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: human
• Reattività contro le specie (prevista in base all’omologia): mouse (based on 100% sequence homology), rat (based on 100% sequence homology), zebrafish (based on 100% sequence homology), sheep (based on 100% sequence homology)
• tecniche: dot blot: suitable; inhibition assay: suitable (peptide); western blot: suitable
• N° accesso NCBI: NP_004949
• N° accesso UniProt: P42345
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: phosphorylation (pSer2159)
• Informazioni sul gene: human ... MTOR(2475)

Descrizione

Descrizione generale

The mammalian target of rapamycin (mTOR), also known as FRAP, RAFT, or RAPT, is a ubiquitously expressed serine/threonine kinase that belongs to the phosphoinositide 3-kinase–related kinase (PIKK) family. mTOR functions as a downstream effector in the PI3K pathway and is activated by the Akt kinase. mTOR responds to signals from growth factors and promotes protein synthesis underlying cell proliferation, by activation of the S6 kinase and inhibition of the 4E-BP protein involved in protein translation. Several studies have suggested that the proliferative functions of mTOR may contribute to the development of cancer.

Specificità

This antibody recognizes mTOR phosphorylated at Ser2159.

Immunogeno

Epitope: Phosphorylated at Ser2159

KLH-conjugated linear peptide corresponding to human mTOR phosphorylated at Ser2159.

Applicazioni

Anti-mTOR Antibody detects level of phospho-mTOR (Ser2159) & has been published & validated for use in Western Blotting, Peptide Inhibition Assay, Dot Blot.

Research Category
Signaling

Research Sub Category
Kinases & Phosphatases

Western Blot Analysis: A representative lot detected phospho-mTOR (Ser2159) in HEK293 cell lysate. Image courtesy from the laboratory of Dr. Diane Fingar, University of Michigan Medical School.

Qualità

Evaluated by Dot Blot in unmodified and modified mTOR peptides.

Dot Blot Specificity Analysis: Unmodified and modified mTOR peptides (see Table) were probed with Anti-phospho-mTOR (Ser2159) (1:1,000 dilution).

Descrizione del bersaglio

290 kDa calculated

Stato fisico

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
Unmodified and modified mTOR peptides

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable