ABS1605

Anti-Cytochrome p450 2J Family Antibody

serum, from rabbit

• Sinonimo/i: Cytochrome P450 2J, Arachidonic acid epoxygenase, CYP2J, CYPIIJ, Cytochrome P450 subfamily IIJ polypeptide, Flavoprotein-linked monooxygenase, Microsomal monooxygenase
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: serum
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Reattività contro le specie: human, rat, mouse
• Reattività contro le specie (prevista in base all’omologia): porcine (based on 100% sequence homology), sheep (based on 100% sequence homology)
• tecniche: immunohistochemistry: suitable (paraffin); western blot: suitable
• N° accesso NCBI: NP_000766
• N° accesso UniProt: P51589
• Condizioni di spedizione: dry ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... CYP2J2(1573)

Descrizione

Descrizione generale

Cytochrome P450 2J2 (EC 1.14.14.1; UniProt P51589; also known as Arachidonic acid epoxygenase, CYPIIJ2, Cytochrome P450 subfamily IIJ polypeptide 2, Flavoprotein-linked monooxygenase, Microsomal monooxygenase) is encoded by the CYP2J2 (also known as CPJ2) gene (Gene ID 1573) in human. Cytochromes P450 (CYP) proteins are primarily membrane-associated oxidases located either in the inner membrane of mitochondria or in the endoplasmic reticulum where they function as the terminal enzymes in electron transfer chains. In addition to processing endogenous substrates, CYPs also function to metabolize exogenous drugs and potentially toxic chemicals. The human CYP superfamily consisits of 57 genes and more than 59 pseudogenes divided into 18 families and 43 subfamilies. The CYP2J subfamily of enzymes are responsible for the epoxidation of endogenous arachidonic acid (AA) into epoxyeicosatrienoic acids (EETs) and 20-hydroxyeicosatetraenoic acid (20-HETE).

Specificità

This polyclonal antiserum is also known as Anti-CYP2J2pep3. It cross-reacts with all known CYP2J subfamily P-450s, but not non-CYP2J subfamily P-450s (Yaghi, A., et al. (2003). Am. J. Physiol. Lung Cell Mol. Physiol. 285(5): L1099–L1105).

Immunogeno

Epitope: Internal (C-terminal half).

Linear peptide corresponding to an internal sequence from the C-terminal half of human cytochrome p450 2J2.

Applicazioni

Anti-Cytochrome p450 2J Family Antibody is an antibody against Cytochrome p450 2J Family for use in Western Blotting
, Immunohistochemistry (Paraffin).

Immunohistochemistry Analysis: An 1:8,000 dilution from a representative lot detected cardiomyocytes with overexpressed human CYP2J2 in formalin-fixed, paraffin-embedded heart tissue sections from transgenic mice carrying human CYP2J2 transgene downstream of αMHC promoter to allow cardiomyocyte-specific human CYP2J2 expression (Courtesy of Dr. Darryl C. Zeldin, NIEHS, NIH, Research Triangle Park, NC).
Western Blotting Analysis: An 1:2,000 dilution from a representative lot detected recombinant CYP2J (2J2, 2J3, 2J5, 2J8, 2J9, 2J12, 2J13), but not CYP2C (2C44, 2C50, 2C54) proteins (Courtesy of Dr. Darryl C. Zeldin, NIEHS, NIH, Research Triangle Park, NC).
Western Blotting Analysis: A representative lot detected the overexpressed human CYP2J2 in primary lung endothelial cells (ECs) from transgenic mice carrying human CYP2J2 transgene downstream of the murine Tie2 promoter to allow endothelial expression of human CYP2J2 (Deng, Y., et al. (2011). FASEB J. 25(2):703–713).
Western Blotting Analysis: A representative lot detected a 51 kDa CYP2J band in MIH-2 mouse hepatocellular carcinoma cells (HCCs), but not in C26 and MC38 mouse colon cancer cells or T3 mouse small intestine cancer cells (Homma, S., et al. (2009). Clin. Exp. Immunol. 156(2):344-352).
Western Blotting Analysis: A representative lot detected different CYP2J banding patterns between prenatal and postnatal human liver microsomal samples from human subjects ranging from 1.1 EGA (estimated gestational age) to 17-year-old of age (Gaedigk, A., et al. (2006). J. Pharmacol. Exp. Ther. 319(2): 523–532).
Western Blotting Analysis: A representative lot detected the overexpressed human CYP2J2 in heart tissues from transgenic mice carrying human CYP2J2 transgene downstream of αMHC promoter to allow cardiomyocyte-specific transgene expression (Seubert, J., et al. (2004). Circ. Res. 95(5):506-514).
Western Blotting Analysis: A representative lot detected a reduced CYP2J level in lung microsome preparations from pneumonia rats when compared with control rats (Yaghi, A., et al. (2003). Am. J. Physiol. Lung Cell Mol. Physiol. 285(5): L1099–L1105).
Western Blotting Analysis: A representative lot detected recombinant human (2J2), rat (2J3 & 2J4), and mouse (2J5, 2J6, 2J8, 2J9) CYP2J proteins (Yaghi, A., et al. (2003). Am. J. Physiol. Lung Cell Mol. Physiol. 285(5): L1099–L1105).
Immunohistochemistry Analysis: A representative lot detected the overexpressed human CYP2J2 in formalin-fixed, paraffin-embedded heart tissue sections from transgenic mice carrying human CYP2J2 transgene downstream of αMHC promoter to allow cardiomyocyte-specific transgene expression (Seubert, J., et al. (2004). Circ. Res. 95(5):506-514).

Research Category
Signaling

Research Sub Category
Enzymes & Biochemistry

Qualità

Evaluated by Western Blotting in rat liver tissue lysate.

Western Blotting Analysis: An 1:5,000 dilution of this antibody detected cytochrome p450 2J protein in 10 µg of rat liver tissue lysate.

Descrizione del bersaglio

~50 kDa observed.

Stato fisico

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Stoccaggio e stabilità

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable