ABS983

Anti-DMT1 Antibody

serum, from rabbit

• Sinonimo/i: NRAM1, NRAMP 2, NRAMP-2, Divalent metal transporter 1, Divalent cation transporter 1, DMT-1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: serum
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Reattività contro le specie: mouse, human
• tecniche: immunocytochemistry: suitable; western blot: suitable
• N° accesso NCBI: NP_000608
• N° accesso UniProt: P49281
• Condizioni di spedizione: dry ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... SLC11A2(4891)

Descrizione

Descrizione generale

Divalent metal ion transporter 1 (DMT1) is a proton-coupled Fe2+ transporter that is essential for iron uptake in enterocytes and for transferrin-associated endosomal iron transport in many other cell types. DMT1 dysfunction is associated with several diseases such as iron overload disorders and neurodegenerative diseases. DMT1 can transport manganese, cobalt, cadmium, nickel, and vanadium. DMT1 is also involved in iron transport from acidified endosomes into the cytoplasm in erythroid precursor cells as well as liver internal metabolism of iron. Interestingly too, in mammalian cells, mitochondria receive most incoming iron, and the outer mitochondrial membrane appears to be a major location for DMT1, which appears to be the major importer of iron into the mitochondria.

Immunogeno

Recombinant protein corresponding to human DMT1.

Applicazioni

Research Category
Signaling

Metabolism

Research Sub Category
Ion & Transport Channels

Ion Channels & Transporters

This Anti-DMT1 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of DMT1.

Western Blot Analysis: 2 µg/ml of this antibody detected DMT1 in 50 µg of mouse liver, spleen and duodenum tissue lysate (Schumann, K.,et al. (2010). Journal of Trace Elements in Medicine and Biology 24 (2010) 58–66)
Immunocytochemistry Analysis: A 1:100 dilution of this antibody from a representative lot detected DMT1 in Hela, A431, and N1H-3T3 cells

Qualità

Evaluated by Western Blotting in human placenta tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected DMT1 in 10 µg of human placenta tissue lysate.

Descrizione del bersaglio

~ 62 kDa observed

Stato fisico

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Stoccaggio e stabilità

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1