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ABN426

Sigma-Aldrich

Anti-phospho-Neurogranin (Ser36)/Neuromodulin (Ser41) Antibody

serum, from rabbit

Sinonimo/i:

Protein kinase C substrate 7.5 kDa protein RC3

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

rabbit

Livello qualitativo

Forma dell’anticorpo

serum

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Reattività contro le specie

mouse

Reattività contro le specie (prevista in base all’omologia)

rat (based on 100% sequence homology)

tecniche

western blot: suitable

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

phosphorylation (pSer36)

Informazioni sul gene

mouse ... Nrgn(64011)
rat ... Nrgn(64356)

Descrizione generale

Neurogranin (Ng) (also named RC3, p17 or BICKS) is a small protein originally identified in rat brain and abundantly expressed in several telencephalic areas, such as the cerebral cortex, hippocampus, amygdala, and striatum. In neurons, it is found concentrated at dendritic spines where it participates in synaptic signaling events through the regulation of calmodulin (CaM). Neurogranin protein is a critical third messenger and substrate of PKC in the molecular cascade necessary for synaptic development and remodeling. Neurogranin (Ng) features an IQ motif that mediates its interaction with CaM and phosphatidic acid (PA). The interaction is controlled by phosphorylation at serine 36 of Neurogranin. Neurogranin is phosphorylated at serine 36 by PKC. Ser36-phosphorylated Neurogranin is unable to bind either CaM or PA. Neurogranin in localized in the cell bodies of neurons in the cortex and in the apical and basal dendrites of pyramidal neurons. Neurogranin is not found in dendrites and its expression is very low as well in Alzheimer’s disease patients.

Specificità

Phosphorylated Neurogranin and Neuromodulin

Immunogeno

Epitope: IQ domain
KLH-conjugated linear peptide corresponding to the IQ domain of Rat phospho-Neurogranin.

Applicazioni

Research Category
Neuroscience
Research Sub Category
Neuroregenerative Medicine
This Anti-phospho-Neurogranin (Ser36)/Neuromodulin (Ser41) Antibody is validated for use in Western Blotting for the detection of phospho-Neurogranin (Ser36)/Neuromodulin (Ser41).

Qualità

Evaluated by Western Blotting in Mouse brain tissue lysate.

Western Blotting Analysis: A 1:1000 dilution of this antibody detected phospho-Neurogranin (Ser36) / Neuromodulin (Ser41) in 10 µg of Mouse brain tissue lysate treate with magnisium (lane1) or PKC (lane2)

Descrizione del bersaglio

~43 kDa is phospho-Neuromodulin (GAP-43) and ~17 KD is phospho-Neurogranin. Uncharacterized band(s) may appear in some lysates.

Stato fisico

Rabbit Polyclonal serum with 0.05% sodium azide.
Unpurified

Stoccaggio e stabilità

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Nikolaus A Watson et al.
Nature communications, 11(1), 1684-1684 (2020-04-05)
There are thousands of known cellular phosphorylation sites, but the paucity of ways to identify kinases for particular phosphorylation events remains a major roadblock for understanding kinase signaling. To address this, we here develop a generally applicable method that exploits

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