ABN1455

Anti-NKX6.2

from rabbit, purified by affinity chromatography

• Sinonimo/i: Homeobox protein NK-6 homolog B, Homeobox protein Nkx-6.2, NKX6.2
• Codice UNSPSC: 12352203
• eCl@ss: 32160702

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: human
• Reattività contro le specie (prevista in base all’omologia): mouse (based on 100% sequence homology), rat (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)
• tecniche: immunohistochemistry: suitable (paraffin); western blot: suitable
• N° accesso NCBI: NP_796374
• N° accesso UniProt: Q9C056
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... NKX6-2(84504)

Descrizione

Descrizione generale

Homeobox protein Nkx-6.2 (UniProt Q9C056; also known as Homeobox protein NK-6 homolog B) is encoded by the NKX6-2 (also known as GTX, NKX6B) gene (Gene ID 84504) in human. Mammalian NKX gene products and their orthologs in other species are known to regulators of neuronal cell fate. Both the ventral nervous system defective (vnd) gene product in Drosophila and murine NKX proteins in spinal cord direct progenitors to adopt specific ventral neural identities. In mice, NKX6.2 is expressed in a small subset of ventral neural progenitors in a restricted spatial pattern at the border between the medial and caudal ganglionic eminence (MGE and CGE). NKX6,2 is required for specification of V1 interneurons in the spinal cord and progenitors are found to adopt a more dorsal V0 identity in the absence of NKX6,2. In addition, NKX6.2-positive brain region is shown to give rise to both parvalbumin (PV) and somatostatin (SST) cortical interneurons and in particular be enriched in a subpopulation of SST cortical interneurons that coexpress calretinin (CR).

Specificità

This rabbit polyclonal antibody detected the exogenously expressed human NKX6.2 in transfected HEK293T cells, while no immunoreactive bands were detected in lysates from untransfected cells. The immunogen sequence lies within the region C-terminal to the Homeobox and is 100% conserved among human, mouse, and rat species.

Immunogeno

KLH-conjugated linear peptide corresponding to a C-terminal region sequence of human/mouse/rat NKX6.2.

Applicazioni

Anti-NKX6.2, Cat. No. ABN1455, is a highly specific rabbit polyclonal antibody that targets Homeobox protein Nkx-6.2 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected NKX6.2 in human cerebral cortex and kidney tissue sections.

Western Blotting Analysis: 1 µg/mL from a representative lot detected Myc-DDK-tagged human NKX6.2 in 10 µg of lysate from transfected HEK293T cells.

Research Category
Neuroscience

Qualità

Evaluated by Immunohistochemistry in human pancreas tissue.

Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected NKX6.2 in human pancreas tissue section.

Descrizione del bersaglio

~29 kDa observed. 29.26 kDa (human) and 29.36 kDa (mouse and rat) calculated. Uncharacterized bands may be observed in some lysate(s).

Stato fisico

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable