ABE569
Anti-monoubiquitin H2A Antibody (Lys119)
from rabbit, purified by affinity chromatography
Sinonimo/i:
H2AK119ub1, Histone H2A.1, Histone H2A.2, H2A2, h2aUb
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
rabbit
Livello qualitativo
Forma dell’anticorpo
affinity isolated antibody
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Purificato mediante
affinity chromatography
Reattività contro le specie
human, mouse
tecniche
ChIP: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
rat ... Hist2H2Aa2(690131)
Descrizione generale
Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The ubiquitination of Histones plays essential roles in the regulation of DNA related processes.
Immunogeno
Branch peptide corresponding to human monoubiquitin H2A (Lys119).
Applicazioni
Detect Histone H2A using this rabbit polyclonal antibody, Anti-monoubiquitin H2A Antibody (Lys119) validated for use in western blotting, ChIP, IP and ICC.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Western Blotting Analysis: A representative lot from an independent laboratory detected monoubiquitin H2A in mouse Embryonic Stem cell lysate (Farcas, A. M. et al. (2012). Elife [Epub ahead of print].).
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated monoubiquitin H2A in mouse Embryonic Stem cell lysate (Farcas, A. M. et al. (2012). Elife [Epub ahead of print].).
Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated monoubiquitin H2A in mouse Embryonic Stem cell lysate (Farcas, A. M. et al. (2012). Elife [Epub ahead of print].).
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated monoubiquitin H2A in mouse Embryonic Stem cell lysate (Farcas, A. M. et al. (2012). Elife [Epub ahead of print].).
Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated monoubiquitin H2A in mouse Embryonic Stem cell lysate (Farcas, A. M. et al. (2012). Elife [Epub ahead of print].).
Qualità
Evaluated by Western Blotting in HeLa acid extract.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected monoubiquitin H2A (Lys119) in 10 µg of HeLa acid extract.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected monoubiquitin H2A (Lys119) in 10 µg of HeLa acid extract.
Descrizione del bersaglio
~23 kDa observed. Uniprot describes one isoform at 14 kDa; however, this protein may be observed at ~23 kDa due to monoubiquitination.
Stato fisico
Affinity purified
Purified rabbit polyclonal in buffer containing 90 mM Tris-glycine pH8, 50 mM Nacl, and 10% glycerol with 0.05% sodium azide
Stoccaggio e stabilità
Stable for 1 year at 2-8°C from date of receipt.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Risultati analitici
Control
HeLa acid extract
HeLa acid extract
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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