ABE23

Anti-Gdown1 Antibody

from sheep, purified by affinity chromatography

• Sinonimo/i: Myocardial zonula adherens protein, GRINL1A upstream protein, Gup, Gdown1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: sheep
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: human
• tecniche: ChIP: suitable; western blot: suitable
• Isotipo: IgG
• N° accesso NCBI: NP_001018101
• N° accesso UniProt: P0CAP1
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... POLR2M(81488)

Descrizione

Descrizione generale

Gdown1 (DNA-directed RNA polymerase II subunit GRINL1A; DNA-directed RNA polymerase II subunit M; glutamate receptor-like protein 1A) is a nuclear protein belonging to the GRINL1 family. It binds to the RNA Polymerase II (G) molecule (RNAPII (G)) and prevents its interaction with transactivating factors, thereby suppressing RNAPII (G)-mediated transcriptional events. Gdown1 also indirectly suppresses RNAPII (G)-transcription by blocking the binding of the elongation-enhancing factor TFIIF to the elongation complex. Gdown1 also prevents TFIIF-mediated assembly of the preinitiation complex required for recruiting RNAPII to the promoter region, by binding competitively to Pol II at the RPB1 and RPB5 subunits recognized by TFIIF. Gdown1 may also inhibit the termination factor TTF2. The effects of Gdown1 are blocked by the P-TEFb. Overall, Gdown1 regulates the transcriptional activity of RNAPII (G) at multiple promoter regions by various mechanisms. Gdown1 has been detected in brain, testis, prostate, lung, small intestine, heart, kidney, and skeletal muscle.

Immunogeno

Histidine-tagged recombinant protein corresponding to human Gdown1.

Applicazioni

Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in HeLa cells. (Price, D., et al. (2012). Cell. 45(1):38-50.)

Chromatin Immunoprecipitation Sequencing Analysis: A representative lot was used by an independent laboratory in HeLa cells. (Price, D., et al. (2012). Cell. 45(1):38-50.)

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Metabolism & Binding Proteins

Use Anti-Gdown1 Antibody (Sheep Polyclonal Antibody) validated in WB, ChIP to detect Gdown1 also known as Myocardial zonula adherens protein, GRINL1A upstream protein, Gup.

Qualità

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Gdown1 on 10 µg of HeLa cell lysate.

Descrizione del bersaglio

~50 kDa observed. Uniprot describes 11 isoforms ranging between 44 and 64 kDa

Stato fisico

Affinity purified

Purified sheep polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
HeLa cell lysate

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable