ABE1797

Anti-Transcription Initiation Factor IIA subunit 1

from rabbit, purified by affinity chromatography

• Sinonimo/i: General transcription factor IIA subunit 1, TFIIAL, Transcription initiation factor TFIIA 42 kDa subunit, TFIIA-42
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: human
• Reattività contro le specie (prevista in base all’omologia): mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
• Confezionamento: antibody small pack of 25 μg
• tecniche: immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotipo: IgG
• N° accesso NCBI: NP_056943
• N° accesso UniProt: P52655
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... GTF2A1(2957)

Descrizione

Descrizione generale

Transcription initiation factor IIA subunit 1 (UniProt: P52655; also known as General transcription factor IIA subunit 1, TFIIAL, Transcription initiation factor TFIIA 42 kDa subunit, TFIIA-42) is encoded by the GTF2A1 (also known as TF2A1) gene (Gene ID: 2957) in human. TFIIA is one of the several components of the transcription machinery of RNA polymerase II that plays an important role in transcriptional activation. TFIIA in a complex with TATA-box-binding protein (TBP) mediates transcriptional activity. After initiation, some components of the pre-initiation complex, including TFIIA, remain attached and re-initiate a subsequent round of transcription. Binding of TFIIA to TBP helps to stabilize TBP binding to the TATA element. TFIIA is a heterodimer of the large unprocessed subunit 1 and a small subunit gamma. It was originally believed to be a heterotrimer of an alpha (p35), a beta (p19) and a gamma subunit (p12).

Specificità

This rabbit polyclonal antibody detects Transcription initiation factor IIA subunit 1 in human cells. It targets an epitope within 15 amino acids from the C-terminal region.

Immunogeno

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human Transcription initiation factor IIA subunit 1.

Applicazioni

Anti-Transcription Initiation Factor IIA subunit 1, Cat. No. ABE1797, is a highly specific rabbit polyclonal antibody that targets Transcription initiation factor IIA subunit 1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Research Category
Epigenetics & Nuclear Function

Western Blotting Analysis: 0.2 µg/mL from a representative lot detected Transcription initiation factor IIA subunit 1 in 10 µg of RAW264.7 cell lysate.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Transcription initiation factor IIA subunit 1 in human brain and human testis tissue.

Qualità

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected Transcription initiation factor IIA subunit 1 in 10 µg of HeLa cell lysate.

Descrizione del bersaglio

~55 kDa observed; 41.51 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Stato fisico

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): does not flash
• Punto d’infiammabilità (°C): does not flash