ABC1454M

Anti-Sodium-Iodide Symporter Antibody, C-terminal 2

from rabbit

• Sinonimo/i: Sodium/iodide cotransporter, Na(+)/I(-) cotransporter, Na(+)/I(-) symporter
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Reattività contro le specie: human
• tecniche: flow cytometry: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• Isotipo: IgG
• N° accesso NCBI: NP_000444
• N° accesso UniProt: Q92911
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... SLC5A5(6528)

Descrizione

Descrizione generale

Sodium/iodide cotransporter (UniProt: Q92911; also known as Na(+)/I(-) cotransporter, Sodium-iodide symporter, Na(+)/I(-) symporter) is encoded by the SLC5A5 (also known as NIS) gene (Gene ID: 6528) in human. Sodium/iodide cotransporter is a multi-pass membrane protein that mediates iodide uptake in thyroid gland. It is expressed primarily in thyroid tissue, but is present in lower levels in mammary gland and ovary. It expression is significantly reduced in tumors. The presence of symporter in mammary glands leads to secretion of iodine in milk that is required for thyroid function in neonatal animals. NIS gene and protein expression is stimulated by thyroid-stimulating hormone. Both human and rat NIS contain N-glycosylation sites, however, glycosylation is not required for its full activity. Abnormalities in the expression of NIS gene lead to thyroid disease and can result in congenital hypothyroidism. Autoantibodies to NIS adversely affect iodide transport.

Specificità

This rabbit polyclonal antibody detects Sodium-iodide symporter in human. It targets an epitope within 13 amino acids from the C-terminal end. For Western blotting application, please do not boil the sample to avoid any aggregation. When transfering protein to nitrocellulose membrane use of 0.05% (w/v) lithium dodecyl sufate is highly recommended in the transfer buffer.

Immunogeno

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to 13 amino acids from the C-terminal region of human Sodium-iodide symporter.

Applicazioni

Anti-Sodium-Iodide Symporter, C-terminal 2, Cat. No. ABC1454, is a rabbit polyclonal antibody that detects Sodium/iodide cotransporter and has been tested for use in Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected Sodium-Iodide Symporter in human thyroid and human salivary gland tissue sections.

Immunocytochemistry Analysis: A representative lot detected Sodium-Iodide Symporter in COS-7 cells transfected with wild-type human NIS and in COS-7 cells transfected with mutant Q267E human NIS (De la Vieja, A., et. al. (2005). Mol Endocrinol. 19(11):2847-58; De la Vieja, A., et. al. (2004). J Cell Sci. 117(Pt 5):677-87).

Immunohistochemistry Analysis: A representative lot detected Sodium-Iodide Symporter in Immunohistochemistry application (De la Vieja, A., et. al. (2004). J Cell Sci. 117(Pt 5):677-87; Wapnir, I.L., et. al. (2004). Clin Cancer Res. 10(13):4294-302; Wapnir, I.L., et. al. (2003). J Clin Endocrinol Metab. 88(4):1880-8).

Immunoprecipitation Analysis: A representative lot detected Sodium-Iodide Symporter in Biotinylated NIS constructs expressed in COS-7 cells (De la Vieja, A., et. al. (2004). J Cell Sci. 117(Pt 5):677-87).

Flow Cytometry Analysis: A representative lot detected Sodium-Iodide Symporter in COS-7 cells transfected with WT and mutant Q267E NIS (De la Vieja, A., et. al. (2004). J Cell Sci. 117(Pt 5):677-87).

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Sodium-Iodide Symporter in COS-7 cells transfected with hNIS (Courtesy of Dr Nancy Carrasco & Dr. Andrea Reyna-Neyra at Yale University).

Western Blotting Analysis: A representative lot detected Sodium-Iodide Symporter in COS-7 cells transfected with NIS and COS-7 cells transfected with WT and mutant Q267E NIS (De la Vieja, A., et. al. (2005). Mol Endocrinol. 19(11):2847-58; De la Vieja, A., et. al. (2004). J Cell Sci. 117(Pt 5):677-87).

Immunohistochemistry Analysis: A representative lot detected Sodium-Iodide Symporter in IHC-P human salivary gland and breast tissues (Tazebay, U.H., et. al. (2000). Nat Med. 6(8):871-8).

Research Category
Apoptosis & Cancer

Qualità

Evaluated by Immunohistochemistry in human stomach tissue sections.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Sodium-Iodide Symporter in human stomach tissue sections.

Descrizione del bersaglio

68.66 kDa calculated.

Stato fisico

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 76 mM Tris, 192 mM glycine, pH 7.4 with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable