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Key Documents

AB5312

Sigma-Aldrich

Anti-Growth Associated Protein 43 Antibody

serum, Chemicon®

Sinonimo/i:

GAP-43, Neuromodulin, B-50

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

rabbit

Livello qualitativo

Forma dell’anticorpo

serum

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Reattività contro le specie

mouse, human, monkey, rat, feline, bovine

Produttore/marchio commerciale

Chemicon®

tecniche

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

dry ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... GAP43(2596)

Descrizione generale

GAP-43, a common marker of differentiating neurons, is expressed at elevated levels by developing or regenerating neurons during axon growth. While GAP-43 is an integral membrane protein associated with the cytoplasmic surface of axonal growth cones (and synapses), it is absent from dendritic growth cones. In adult brain, GAP-43 is found in high concentration in presynaptic areas where memory formation is thought to occur, such as frontal cortex, limbic system and hippocampus. Phosphorylation of GAP-43 by PKC (at Ser41 on human, mouse, rat, and bovine GAP-43 or Ser42 on chicken and Xenopus GAP-43) is specifically correlated with certain forms of synaptic plasticity.

Specificità

AB5312 specifically recognizes GAP-43 regardless of the protein′s phosphorylation state. This antiserum has been refered to in the literature as CG-1.

Immunogeno

Purified GAP-43 from feline brain (McIntosh and Parkinson, 1990).

Applicazioni

Immunocytochemistry: 1:100-1:400 for mouse primary neurons, NTera2 neurons.

Immuohistochemistry: 1:2,000 on coronal sections of adult visual cortex (McIntosh and Parkinson, 1990). AB5312 has been used to stain GAP-43 in 4% paraformaldehyde-fixed paraffin-embedded tissue sections of mouse olfactory bulb (Legrier, 2001).

Western blot: 1:500 - 1:1,000. Detects a single band at approximately 50 kDa on western blots of protein extracts from adult visual cortex (McIntosh and Parkinson, 1990).

Immunoprecipitation

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Neuroregenerative Medicine

Signaling Neuroscience
This Anti-Growth Associated Protein 43 Antibody is validated for use in IC, IH, IH(P), IP, WB for the detection of Growth Associated Protein 43.

Descrizione del bersaglio

23.6 kDa

Stato fisico

Serum. Liquid with 0.02% sodium azide added as a preservative.
Unpurified

Stoccaggio e stabilità

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Risultati analitici

Control
Rat dorsal root ganglion tissue that has been subjected to a spinal nerve ligation, cultured neurons

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Histopathological substrate for chronic atrial fibrillation in humans.
Nguyen, BL; Fishbein, MC; Chen, LS; Chen, PS; Masroor, S
Heart Rhythm : the Official Journal of the Heart Rhythm Society null
Age-related changes in cell density and the proliferation rate of olfactory ensheathing cells in the lamina propria of postnatal mouse olfactory mucosa.
Kenta Watanabe, Kenji Kondo, Naonobu Takeuchi, Ken-ichi Nibu, Kimitaka Kaga
Brain Research null
GAP-43 in the cat visual cortex during postnatal development.
McIntosh, H, et al.
Visual Neuroscience, 4, 585-593 (1990)
Polysialic acid limits choline acetyltransferase activity induced by brain-derived neurotrophic factor.
Burgess, Alison and Aubert, Isabelle
Journal of Neurochemistry, 99, 797-806 (2006)
GAP-43 in adult visual cortex.
McIntosh, H and Parkinson, D
Brain Research, 518, 324-328 (1990)

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