AB5018

Anti-Glutamate (No Glutaraldehyde) Antibody

Chemicon^®, from rabbit

• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Reattività contro le specie (prevista in base all’omologia): all
• Produttore/marchio commerciale: Chemicon^®
• tecniche: immunohistochemistry: suitable
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified

Descrizione

Specificità

Glutamate (L-Glutamate). The antibody has been calibrated against a spectrum of antigens to assure hapten selectivity and proper affinity. No measurable cross-reactivity (<1:1000) against glutamate in peptides or proteins. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) against L-alanine, gamma-aminobutyrate, 1-amino-4-guanidobutane (AGB), D/L-arganine, D/L-aspartate, L-citrulline, L-cysteine, D/L-glutamine, glutathione, glycine, L-lysine, L-ornithine, L-serine, taurine, L-threonine, L-tryptophan, L-tyrosine. Modest cross-reactivity (1:20) against D-glutamate. Significant cross-reactivity (1:8) against free NAAG in competition assays (NAAG is not retained in glutaraldehyde-fixed tissue).

Immunogeno

Glutamate-glutaraldehyde

Applicazioni

Immunohistochemistry using silver-intensified immunogold or fluorescence (see recommended protocol). Samples should be fixed with 0.1% - 2.5% glutaraldehyde for optimum detection.

*This antibody has also been used and found to work with a zero-low glutaraldehyde / high paraformaldehyde fixation (4% paraformaldehyde in 0.1M phosphate buffer / 3% sucrose fixative ). The minimum glutaraldehyde concentration for AB5018 is 0.00%. See protocol that follows. Performance is good with frozen sections, Vibratome sections and tissue culture formats, when penetrating reagents such as 0.3% Triton X-100 are used.

Optimal working dilutions must be determined by the end user.

DILUTION: Prepare enough of the AB5018 for your days use by diluting 100X with 1% GSPBT.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Neuroinflammation & Pain

This Anti-Glutamate (No Glutaraldehyde) Antibody is validated for use in IH for the detection of Glutamate (No Glutaraldehyde).

Stato fisico

Format: Purified

IgG fraction in sterile 0.1M phosphate buffer. No preservative.

Stoccaggio e stabilità

Maintain stock at 2-8°C in undiluted aliquots for up to 6 months. This stock is extremely stable under normal use and routine storage at 2-8°C. Do not freeze.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Indicazioni di pericolo: H412
• Consigli di prudenza: P273 - P501
• Classi di pericolo: Aquatic Chronic 3
• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°C): Not applicable