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Key Documents

AB3202

Sigma-Aldrich

Anti-LIM-3 Antibody

Chemicon®, from rabbit

Sinonimo/i:

Anti-HMFN1661

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

rabbit

Livello qualitativo

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Reattività contro le specie

fish, frog, mouse, human

Produttore/marchio commerciale

Chemicon®

tecniche

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... ABLIM3(22885)

Specificità

Recognizes LIM-3. This antibody does not appear to cross react with gsh-4 on tissue sections but may cross react in Western blot and immunoprecipitation.

Immunogeno

C-terminal portion of mouse LIM-3 protein.

Applicazioni

Anti-LIM-3 Antibody is an antibody against LIM-3 for use in IP, WB, IC, IH, IH(P).
Immunohistochemistry: 1:250-1:1,000 on paraffin embedded sections. 1:3,000-1:6,000 on frozen sections. 1:4,000-1:8,000 on whole mounts. Recommended fixative MEMFA.

Immunocytochemistry: 1:200-1:500

Western blot: 1:2,500-1:5,000

Immunoprecipitation: 1:200

This antibody will work well in immunofluorescence. Use a secondary antibody conjugated to biotin and then streptavidin-conjugated to fluorochrome.

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience

Neuronal & Glial Markers

Stato fisico

Format: Purified
Purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.

Stoccaggio e stabilità

Maintain at 2-8°C in undiluted aliquots for up to 6 months.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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The LIM-homeodomain proteins Isl-1 and Lhx3 act with steroidogenic factor 1 to enhance gonadotrope-specific activity of the gonadotropin-releasing hormone receptor gene promoter.
Granger, A; Bleux, C; Kottler, ML; Rhodes, SJ; Counis, R; Laverriere, JN
Molecular Endocrinology null
Mai Nakamura et al.
Cytotechnology, 68(3), 409-417 (2014-10-31)
Mouse embryonic stem (ES) cells and induced pluripotent stem (iPS) cells have the ability to differentiate in vitro into various cell lineages including neurons. The differentiation of these cells into neurons has potential applications in regenerative medicine. Previously, we reported
Clifford R Hume et al.
Gene expression patterns : GEP, 7(7), 798-807 (2007-07-03)
A cascade of transcription factors is believed to regulate the coordinate differentiation of primordial inner ear cells into the subtypes of hair cells and supporting cells. While candidate genes involved in this process have been identified, the temporal and spatial
Stephanie W Fowler et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(23), 7871-7885 (2014-06-06)
An unresolved debate in Alzheimer's disease (AD) is whether amyloid plaques are pathogenic, causing overt physical disruption of neural circuits, or protective, sequestering soluble forms of amyloid-β (Aβ) that initiate synaptic damage and cognitive decline. Few animal models of AD
Zhiyong Liu et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 32(19), 6600-6610 (2012-05-11)
Unlike nonmammalian vertebrates, mammals cannot convert inner ear cochlear supporting cells (SCs) into sensory hair cells (HCs) after damage, thus causing permanent deafness. Here, we achieved in vivo conversion of two SC subtypes, pillar cells (PCs) and Deiters' cells (DCs)

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