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Documenti fondamentali

AB10554

Sigma-Aldrich

Anti-Tbr1 Antibody

from rabbit, purified by affinity chromatography

Sinonimo/i:

T-box, brain, 1, T-brain-1, T-box brain protein 1

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

rabbit

Livello qualitativo

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Purificato mediante

affinity chromatography

Reattività contro le specie

mouse

Reattività contro le specie (prevista in base all’omologia)

canine (based on 100% sequence homology), canine, bovine, opossum, horse, rat, pig

tecniche

immunohistochemistry: suitable (paraffin)
western blot: suitable

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... TBR1(10716)

Descrizione generale

T-box brain protein 1 (UniProt: Q64336; also known as T-brain-1, TBR-1, TES-56) is encoded by the Tbr1 gene (Gene ID: 21375) in murine species. Tbr1 is a homodimeric nuclear protein that serves as a transcriptional repressor and is involved in multiple aspects of cortical development, including neuronal migration, laminar and areal identity, and axonal projection. As transcriptional repressor of FEZ Family Zinc Finger 2 (FEZF2), it blocks the formation of the corticospinal (CS) tract from layer 6 projection neurons, thereby restricting the origin of CS axons specifically to layer 5 neurons. Its T-box DNA binding domain is localized in amino acids 213-393. Its expression is observed in developing and adult cerebral cortex and in olfactory bulbs. It is detected around day 10 of embryonic development in the preplate and at day 12.5 in the cortical plate. It is highly expressed in glutamatergic early-born cortical neurons. Tbr1 null mice exhibit severe defects of frontal cortex and layer 6 differentiation. (Ref.: Han, W., et al. (2011). Proc. Natl. Acad. Sci. USA. 108(7); 3041-3046; Bedogni, F., et al (2010). Proc. Natl Acad. Sci. USA. 107(29); 13129-13134; Hevner, RF., et al. (2001). Neuron. 29(2); 353-366).

Specificità

This rabbit polyclonal antibody detects T-box brain protein 1 (Tbr1).

Immunogeno

KLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal region of mouse T-box brain protein 1 (Tbr1).
KLH-conjugated linear peptide corresponding to mouse Tbr1 near the N-terminus.

Applicazioni

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in Mouse brain tissue sections.Immunohistochemistry (Paraffin) Analysis: A 1:400 dilution of this antibody detected Tbr1 in Mouse cerebral cortex and cerebellum tissue sections.


Tested Applications

Western Blotting Analysis: A 1:500 dilution from a representative lot detected Tbr1 in Mouse fetal brain tissue lysate.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Qualità

Evaluated by Immunohistochemistry in mouse frontal cortex tissue.

Immunohistochemistry Analysis: 1:400 dilution of this antibody detected Tbr1 in mouse frontal cortex tissue.

Descrizione del bersaglio

~76 kDa observed; 73.94 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Stato fisico

Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Recommended storage: +2°C to +8°C.

Risultati analitici

Control
Mouse frontal cortex tissue

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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