Passa al contenuto
Merck
Tutte le immagini(1)

Key Documents

View All Documentation

70922

Millipore

BugBuster® HT Protein Extraction Reagent

Sinonimo/i:

Cell lysis reagent, Protein extraction buffer

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Tutte le immagini(1)

About This Item

Codice UNSPSC:
41106511
NACRES:
NA.77

Forma fisica

liquid

Livello qualitativo

100

Produttore/marchio commerciale

Novagen®

Condizioni di stoccaggio

OK to freeze
avoid repeated freeze/thaw cycles

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

Categorie correlate

Descrizione generale

BugBuster HT Protein Extraction Reagent combines BugBuster Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from E. coli without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.

Applicazioni

BugBuster® HT Protein Extraction Reagent has been used:
  • to lyse the Escherichia coli cell pellets following centrifugation for protein expression and immunoblotting
  • for protein extraction from bacterial cell pellet
  • used to lyse the E. coli cell pellets for the expression and purification of dMIC60 (mitofilin) protein

Caratteristiche e vantaggi

  • Fast protein extraction and nucleic acid degradation
  • Ideal for processing various samples of different volumes
  • Increased extraction is ensured by the addition of rLysozyme solution

Attenzione

Toxicity: Standard Handling (A)

Note legali

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

Chiara Pedicone et al.
iScience, 25(4), 104170-104170 (2022-04-26)
Here, we describe the use of artificial intelligence to identify novel agonists of the SH2-containing 5' inositol phosphatase 1 (SHIP1). One of the compounds, K306, represents the most potent agonist identified to date. We find that K306 exhibits selectivity for

Articoli

Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens

Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens

BugBuster® Plus Lysonase™ Kit: Cell lysis and nucleic acid removal for Gram-negative and Gram-positive bacteria

Cell lysis and nucleic acid removal for Gram-negative and Gram-positive bacteria using the BugBuster Plus Lysonase™ Kit

Use of BugBuster® and Lysonase™ Reagents for efficient protein extraction from Gram-positive bacteria

The combination of BugBuster and Lysonase greatly enhances the release of soluble proteins from Gram-positive bacteria.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.