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Sigma-Aldrich

BLR(DE3) Competent Cells - Novagen

Escherichia coli, rod shaped

Sinonimo/i:

Competent cells for cloning

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About This Item

Codice UNSPSC:
41106202
NACRES:
NA.81

product name

BLR(DE3) Competent Cells - Novagen, BLR is a recA derivative of BL21 that improves plasmid monomer yields and may help stabilize target plasmids containing repetitive sequences.

Origine biologica

Escherichia coli

Livello qualitativo

100

Produttore/marchio commerciale

Novagen®

Condizioni di stoccaggio

OK to freeze
avoid repeated freeze/thaw cycles

Modalità di accrescimento

adherent or suspension

Morfologia

rod shaped

tecniche

microbiological culture: suitable

Trasformazione cellulare

transformation efficiency: >2×106 cfu/μg

Condizioni di spedizione

dry ice

Temperatura di conservazione

−70°C

Descrizione generale

Genotype: F-ompT hsdSB(rB- mB-) gal lac ile dcm Δ(srl-recA)i306::Tn10 (tetR)(DE3)





This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
BLR is a recA- derivative of BL21 that improves plasmid monomer yields and may help stabilize target plasmids containing repetitive sequences or whose products may cause the loss of the DE3 prophage (1, 2).

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
BLR is a recA derivative of BL21 that improves plasmid monomer yields and may help stabilize target plasmids containing repetitive sequences.

Componenti

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlBLR(DE3) Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•2 ng2 ngTest Plasmid

Attenzione

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Altre note

1. A. Roca (U. of Wisconsin), personal
communication.

2. Studier, F. W. (1991) J. Mol. Biol. 219,
37-44.

Note legali

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Certificati d'analisi (COA)

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