616407

TACE Substrate IV, Fluorogenic

• Sinonimo/i: TACE Substrate IV, Fluorogenic, TNF-α Convertase Enzyme Substrate IV, Fluorogenic, Abz-LAQAVRSSSR-Dpa, ADAM-17 Substrate IV, Fluorogenic
• Formula empirica (notazione di Hill): C₅₉H₉₃N₂₃O₂₀
• Peso molecolare: 1444.51
• Codice UNSPSC: 12352200
• NACRES: NA.77

Proprietà

• Saggio: ≥95% (HPLC)
• Livello qualitativo: 100
• Stato: lyophilized solid
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; desiccated; protect from light
• Colore: yellow
• Solubilità: DMSO: 5 mg/mL
• Fluorescenza: λ_ex ~320 nm; λ_em ~420 nm
• Condizioni di spedizione: ambient
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

An internally quenched, fluorogenic, 10-amino acid peptide substrate that is useful for monitoring TNF-α Convertase Enzyme (TACE) activity by a continuous fluorometric assay. Also useful for screening TACE inhibitors (k_cat = 21.6 s^-1 when the substrate concentration is half the V_max, S_0.5 = 342 µM). The substrate is cleaved at the Ala-Val amide bond. The substrate is not substantially cleaved by MMP-1, MMP-9, or MMP-13. Displays improved solubility in aqueous buffers. Excitation max: ~320 nm; Emission max: ~420 nm.

An internally-quenched fluorogenic decapeptide substrate that is useful for continuous monitoring of TACE activity and high-throughput screening of TACE inhibitors (k_cat = 21.6 s^-1 and substrate concentration at half the V_max, S_0.5 = 342 µM). The substrate is cleaved at Ala-Val amide bond. Acts as a poor substrate for MMP-1, MMP-9, and MMP-13. Displays better solubility in aqueous buffers.

Azioni biochim/fisiol

Cell permeable: no

Primary Target
Fluorogenic decapeptide substrate that is useful for continuous monitoring of TACE activity

Product does not compete with ATP.

Reversible: no

kcat = 21.6 s-1 and substrate concentration at half the Vmax, S0.5 = 342 µM

Attenzione

Toxicity: Standard Handling (A)

Sequenza

Abz-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Arg-Dpa

Stato fisico

Supplied as a trifluoroacetate salt.

Ricostituzione

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Altre note

Jin, G., et al. 2002. Anal. Biochem.302, 269.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable