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Merck
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Documenti fondamentali

539779

Millipore

ProteoExtract® Complete Mammalian Proteome Extraction Kit

Sinonimo/i:

C-PEK Mammalian Kit

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About This Item

Codice UNSPSC:
41106510
NACRES:
NA.32

impiego

20 extractions

Produttore/marchio commerciale

Calbiochem®

Condizioni di stoccaggio

OK to freeze
avoid repeated freeze/thaw cycles

input

sample type: mammalian tissue(s)
sample type: mammalian cultured cells

Condizioni di spedizione

ambient

Temperatura di conservazione

2-8°C

Descrizione generale

A complete mammalian proteome extraction kit (C-PEK) designed to extract virtually all proteins from both mammalian tissues and culture cells in a simple two step process within a microcentrifuge tube to minimize protein loss. The extraction results in a total protein sample that is a clarified and non-viscous protein solution showing higher resolution and an increased total number of spots in 2-D gel electrophoresis. Sample size: 1x107 - 1x108 cells or 50-100 mg tissue.

Confezionamento

1 kit in Glass bottle

Componenti

Wash Buffer, Resuspension Buffer, Extraction Reagent, Reducing Reagent, Benzonase® Nuclease (Cat. No. 71206), Glass Beads, and a user protocol.

Attenzione

Toxicity: Multiple Toxicity Values, refer to MSDS

Principio

ProteoExtract® Complete Mammalian Proteome Extraction Kit is designed to extract total proteins from mammalian tissue culture cells or mammalian tissue.

Stoccaggio e stabilità

  • Wash Buffer
The Wash Buffer is provided ready-to-use. The buffer should be stored at 4°C.
  • Resuspension Buffer
The Resuspension Buffer is provided ready-to-use. It can be stored at 4°C up to 6 month. For prolonged storage freeze the buffer in convenient aliquots at -20°C.

Avoid repeated freezing and thawing!
  • Extraction Reagent
The Extraction Reagent is supplied as dry powder and can be stored unopened at 4°C. The Extraction Reagent should be reconstituted just prior to use as described below. After reconstitution, it can be stored at 4°C up to 6 month. For prolonged storage freeze the buffers in convenient aliquots at -20°C.

Avoid repeated freezing and thawing!
  • Reducing Agent
The Reducing Agent is supplied as dry powder. It can be stored unopened at 4°C. The Reducing Agent should be reconstituted just prior to use as described below. After reconstitution, aliquot the unused material in 60 µl volumes and freeze at -20°C for further use.

Avoid repeated freezing and thawing!
  • Benzonase®
Benzonase® should be stored at 4°C.

Altre note

Yuan, X., et al. 2002. Electrophoresis 23, 1185. Allen, L. 2000. Functional Genomics Nature 405 819. Herbert, B. 1999. Electrophoresis 20, 660.

Note legali

Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PROTEOEXTRACT is a registered trademark of Merck KGaA, Darmstadt, Germany

Avvertenze

Danger

Indicazioni di pericolo

H302,H315,H318,H351,H361fd,H412

Classi di pericolo

Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2

Codice della classe di stoccaggio

10 - Combustible liquids

Certificati d'analisi (COA)

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