480010-M

NDSB-256

A non-detergent sulfobetaine. Reported to prevent protein aggregation and facilitate the renaturation of chemically and thermally denatured proteins.

• Sinonimo/i: NDSB-256, Dimethylbenzylammonium Propane Sulfonate
• Formula empirica (notazione di Hill): C₁₂H₁₉NO₃S
• Numero CAS: 81239-45-4
• Peso molecolare: 257.35
• Numero MDL: MFCD00225018
• Codice UNSPSC: 12161902
• NACRES: NA.21

Proprietà

• Livello qualitativo: 100
• Saggio: ≥99% (TLC)
• Stato: solid
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; desiccated (hygroscopic)
• Colore: white
• Solubilità: water: 200 mg/mL
• Temperatura di conservazione: 10-30°C
• Stringa SMILE: [S](=O)(=O)([O-])CCC[N+](Cc1ccccc1)(C)C
• InChI: 1S/C12H19NO3S/c1-13(2,9-6-10-17(14,15)16)11-12-7-4-3-5-8-12/h3-5,7-8H,6,9-11H2,1-2H3; MEJASPJNLSQOAG-UHFFFAOYSA-N

Descrizione

Descrizione generale

A member of the class of nondetergent sulfobetaines. Unlike CHAPS and other zwittergents, NDSBs are nondenaturing even at 1 M concentrations. NDSBs have been used to improve the efficiency of solubilization of membrane proteins, to isolate nuclear proteins, to chromatographically purify halophilic proteins in low ionic strength buffers, and to crystallize proteins. They are zwitterionic over a wide pH range and do not absorb significantly in the near UV range. Reported to prevent protein aggregation and facilitate the renaturation of chemically and thermally denatured proteins. NDSBs can be easily removed by dialysis. At 1 M, NDSB-256 will restore 30% of the enzymatic activity of denatured egg white lysozyme; at 800 mM it will restore 16% of the enzymatic activity of denatured β-galactosidase.

A non-detergent sulfobetaine. Reported to prevent protein aggregation and facilitate the renaturation of chemically and thermally denatured proteins. Zwitterionic over a wide pH range. Easily removed by dialysis. At 1 M, NDSB-256 will restore 30% of the enzymatic activity of denatured egg white lysozyme; at 800 mM it will restore 16% of the enzymatic activity of denatured β-galactosidase.

Attenzione

Toxicity: Irritant (B)

Ricostituzione

Following reconstitution, store at room temperature (15-30°C). Stock solutions are stable for up to 3 months at room temperature.

Altre note

Goldberg, M.E., et al. 1996. Folding & Design1, 21.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable