475981

Myosin Light Chain Kinase Inhibitor Peptide 18

The Myosin Light Chain Kinase Inhibitor Peptide 18 controls the biological activity of Myosin Light Chain Kinase. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.

• Sinonimo/i: Myosin Light Chain Kinase Inhibitor Peptide 18, H-RKKYKYRRK-NH₂, MLCK Inhibitor Peptide 18, MLCK Inhibitor IV, MLCK Inhibitor Peptide 18, H-RKKYKYRRK-NH₂, MLCK Inhibitor IV
• Formula empirica (notazione di Hill): C₆₀H₁₀₅N₂₃O₁₁
• Peso molecolare: 1324.62
• Codice UNSPSC: 12352202
• NACRES: NA.77

Proprietà

• Livello qualitativo: 100
• Saggio: ≥97% (HPLC)
• Stato: lyophilized solid
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; desiccated (hygroscopic)
• Colore: white
• Solubilità: water: 1 mg/mL; DMSO: 5 mg/mL
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

A highly basic, cell-permable, nonapeptide that acts as a selective myosin light chain kinase (MLCK) inhibitor (IC₅₀ = 50 nM). Does not block calmodulin (CaM) or inhibit the kinase activities of Ca²⁺/calmodulin kinase II (CaMKII) or protein kinase A (PKA). Inhibition is competitive with respect to substrate (K_i = 52 nM). Displays mixed mode inhibition with respect to ATP.

A highly basic, cell-permeable, nonapeptide that acts as a selective Myosin Light Chain Kinase (MLCK) inhibitor (IC₅₀ = 50 nM). The inhibition is competitive with respect to the peptide substrate (K_i = 52 nM) and displays a mixed mode of inhibition with respect to ATP. Does not interfere with kinase activation by calmodulin (CaM), nor does it inhibit the activities of CaMKII and PKA.

Azioni biochim/fisiol

Cell permeable: yes

Primary Target
Mlck

Product does not compete with ATP.

Reversible: no

Target IC₅₀: 50 nM against MLCK

Confezionamento

Packaged under inert gas

Attenzione

Toxicity: Standard Handling (A)

Sequenza

H-Arg-Lys-Lys-Tyr-Lys-Tyr-Arg-Arg-Lys-NH₂

Stato fisico

Supplied as a trifluoroacetate salt.

Ricostituzione

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Altre note

Zolotarevsky, Y., et al. 2002. Gastroenterology123, 163.
Lukas, T.J., et al. 1999. J. Med. Chem.42, 910.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable