262015

DNA Base Excision Repair Pathway Inhibitor

The DNA Base Excision Repair Pathway Inhibitor, also referenced under CAS 6960-45-8, controls the biological activity of APE1 (human apurinic/apyrimidinic endonuclease.

• Sinonimo/i: DNA Base Excision Repair Pathway Inhibitor, 7-Nitroindole-2-carboxylic acid, CRT0044876
• Formula empirica (notazione di Hill): C₉H₆N₂O₄
• Numero CAS: 6960-45-8
• Peso molecolare: 206.15
• Numero MDL: MFCD00044720
• Codice UNSPSC: 12352200
• NACRES: NA.54

Proprietà

• Livello qualitativo: 100
• Saggio: ≥98% (HPLC)
• Forma fisica: solid
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; protect from light
• Colore: tan
• Solubilità: DMSO: 5 mg/mL
• Condizioni di spedizione: ambient
• Temperatura di conservazione: 2-8°C
• InChI: 1S/C9H6N2O4/c12-9(13)6-4-5-2-1-3-7(11(14)15)8(5)10-6/h1-4,10H,(H,12,13)/p-1; BIUCOFQROHIAEO-UHFFFAOYSA-M

Descrizione

Descrizione generale

A cell-permeable, potent, specific, and nontoxic inhibitor of the DNA repair enzyme, APE1 (human apurinic/apyrimidinic endonuclease; IC₅₀ = ~3 µM). Shown to target the APE1 active site and inhibit its 3′-phosphodiesterase and 3′-phosphatase activities. Exhibits minimal effects on endonuclease IV, BamH1 restriction endonuclease, or topoisomerase I even at concentration as high as 100 µM. Also shown to potentiate the cytotoxicity of several DNA damaging agents, such as MMS, temozolomide, Zeocin, and H₂O₂ in HeLa, HT1080, and MDA-MB-231 cells.

A cell-permeable, potent, specific, and nontoxic inhibitor of the DNA repair enzyme, APE1 (human apurinic/apyrimidinic endonuclease; IC₅₀ ~3 µM). Shown to target the APE1 active site and inhibit its 3′-phosphodiesterase and 3′-phosphatase activities. Exhibits minimal effects on endonuclease IV, BamH1 restriction endonuclease, or topoisomerase I even at concentration as high as 100 µM. Also shown to potentiate the cytotoxicity of several DNA damaging agents, such as MMS, temozolomide, Zeocin, and H₂O₂ in HeLa, HT1080, and MDA-MB-231 cells.

Azioni biochim/fisiol

Cell permeable: yes

Primary Target
APE 1

Product does not compete with ATP.

Reversible: no

Target IC₅₀: ~3 µM against APE1 (human apurinic/apyrimidinic endonuclease

Confezionamento

Packaged under inert gas

Attenzione

Toxicity: Irritant (B)

Ricostituzione

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Altre note

Madhusudan, S., et al. 2005. Nucl. Acids Res.33, 4711.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Pittogrammi: GHS08
• Avvertenze: Danger
• Indicazioni di pericolo: H319,H334
• Consigli di prudenza: P261 - P264 - P280 - P284 - P305 + P351 + P338 - P337 + P313
• Classi di pericolo: Eye Irrit. 2 - Resp. Sens. 1
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable